Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
Bioresour Technol. 2020 Apr;302:122887. doi: 10.1016/j.biortech.2020.122887. Epub 2020 Jan 23.
Immobilization of industrially important enzymes on supports is important to decrease the cost of the overall enzymatic production procedure. Herein, a novel method for synthesizing a new support, magnetic rice straw (MRS) in one step is reported: rice straw (RS) was soaked with Fe ions and these were further reduced to form embedded FeO nanoparticles on the RS surface, forming MRS. This material presented a magnetic saturation value of 27.32 emu g. Lipase immobilization on MRS resulted in 94.3% immobilization efficiency and 91.3 mg g of enzyme loading, which are higher than immobilization on native RS. The lipase stability was increased approximately 8-fold at 70 °C. The lipase-MRS composite was tested in the esterification reaction of biodiesel production, where it showed prominent reusability. Therefore, this novel and rapid synthesis method can provide ecological and economic support for enzyme immobilization and industrially important product formation.
将工业上重要的酶固定在载体上对于降低整个酶生产过程的成本非常重要。本文报道了一种一步法合成新型载体磁性稻草（MRS）的新方法：将稻草（RS）浸泡在 Fe 离子中，然后进一步还原，在 RS 表面形成嵌入式 FeO 纳米颗粒，形成 MRS。该材料的饱和磁化强度为 27.32 emu g。脂肪酶在 MRS 上的固定化，其固定化效率达到 94.3%，酶载量为 91.3 mg g，高于在天然 RS 上的固定化。脂肪酶的稳定性在 70°C 时提高了约 8 倍。脂肪酶-MRS 复合物在生物柴油生产的酯化反应中进行了测试，显示出突出的可重复使用性。因此，这种新颖快速的合成方法可为酶固定化和工业重要产品的形成提供生态和经济支持。
