使用Box-Behnken设计，通过固定在Diaion HP-20上的脂肪酶合成丁酸己酯（苹果和柑橘香气）。
Synthesis of hexyl butyrate (apple and citrus aroma) by lipase immobilized on Diaion HP-20 using the Box-Behnken design.
作者信息
Dos Santos Marta Maria Oliveira, de Menezes Luiz Henrique Sales, do Espirito Santo Eliézer Luz, de Carvalho Marise Silva, Gonçalves Márcia Soares, de Carvalho Tavares Iasnaia Maria, Mendes Adriano Aguiar, Ruiz Héctor A, Salay Luiz Carlos, Franco Marcelo, de Oliveira Julieta Rangel
机构信息
Department of Exact Sciences and Natural, State University of Southwest Bahia, Itapetinga, 45700-000 Brazil.
Department of Exact Sciences and Technology, State University of Santa Cruz, Ilhéus, 45654-370 Brazil.
出版信息
Food Sci Biotechnol. 2022 Nov 30;32(5):689-696. doi: 10.1007/s10068-022-01200-1. eCollection 2023 Apr.
UNLABELLED
This study aims at the synthesis of hexyl butyrate by lipase (CRL) immobilized on Diaion HP 20. The lipase load used was 28.7 ± 2.1 mg/g (mg of lipase/g of support), whose hydrolytic activity was 132.0 ± 2.5 U/g. To obtain the maximum production of hexyl butyrate, the Box-Behnken design statistical planning was used, having as independent variables; biocatalyst concentration, temperature and acid:alcohol molar ratio and ester conversion as a dependent variable at 60, 180 and 480 min. For 60 min, 90.8% conversion was obtained at 47.25 ºC, 1:1.4 molar ratio and 17.65% of biocatalyst; 180 min, 94.5% conversion at 59.5 ºC, 1:2 molar ratio and 15.8% biocatalyst; 480 min, 95.01% conversion at 47.0 ºC, 1:2 molar ratio and 16.9% biocatalyst. CRL-Diaion HP 20 retained 60% of its initial activity after ten cycles of reactions showing potential for industrial use. The ester produced was identified by gas chromatography analyses.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-022-01200-1.
未标记
本研究旨在通过固定在Diaion HP 20上的脂肪酶（CRL）合成丁酸己酯。所用脂肪酶负载量为28.7±2.1 mg/g（每克载体的脂肪酶毫克数），其水解活性为132.0±2.5 U/g。为了获得丁酸己酯的最大产量，采用了Box-Behnken设计统计规划，将生物催化剂浓度、温度和酸：醇摩尔比作为自变量，酯转化率作为60、180和480分钟时的因变量。在60分钟时，在47.25℃、1:1.4摩尔比和17.65%的生物催化剂条件下，转化率为90.8%；180分钟时，在59.5℃、1:2摩尔比和15.8%生物催化剂条件下，转化率为94.5%；480分钟时，在47.0℃、1:2摩尔比和16.9%生物催化剂条件下，转化率为95.01%。CRL-Diaion HP 20在十个反应循环后保留了其初始活性的60%，显示出工业应用潜力。通过气相色谱分析鉴定了产生的酯。
补充信息
在线版本包含可在10.1007/s10068-022-01200-1获取的补充材料。
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