School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Department of Laboratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, PR China; Department of Clinical Laboratory, Zibo Central Hospital, Zibo 255036, PR China.
Bioelectrochemistry. 2020 Jun;133:107461. doi: 10.1016/j.bioelechem.2020.107461. Epub 2020 Jan 30.
Nanomaterials with enzyme properties possess excellent catalytic activity and stability. We prepared new nanoenzymes to construct a label-free electrochemical immunosensor for the detection of hepatitis B e antigen (HBe Ag). In this study, PdCu tripod (PdCu TP) functionalized porous graphene (PG) nanoenzymes (PdCu TPs/PG) were prepared through the in situ reduction of PdCu tripods onto porous graphene. The catalytic Michaelis-Menten kinetic parameters of PdCu TPs/PG are better than horseradish peroxidase (HRP) and show enhanced peroxidase-like activity. Therefore, we used PdCu TPs/PG to catalyse the electrochemically active matrix of HO and generate the synergistically amplified current signal for the subsequent sensitive detection of HBe Ag. Due to the good conductivity, large specific surface area and synergistic amplification of PdCu TPs/PG, the quantitative detection of HBe Ag shows a detection limit of 20 fg·mL and linear range from 60 fg·mL to 100 ng·mL. During the detection of human serum samples, PdCu TPs/PG shows good accuracy based on the standard addition method and a comparison with an ELISA. The prepared immunosensors exhibiting good selectivity, stability and reproducibility provide an important basis for determining the prognosis of hepatitis B and show potential applications in medical applications.
具有酶特性的纳米材料具有优异的催化活性和稳定性。我们制备了新型纳米酶，构建了用于检测乙型肝炎 e 抗原 (HBeAg) 的无标记电化学免疫传感器。在这项研究中，通过将 PdCu 三脚架原位还原到多孔石墨烯上，制备了 PdCu 三脚架功能化的多孔石墨烯纳米酶 (PdCu TPs/PG)。PdCu TPs/PG 的催化米氏动力学参数优于辣根过氧化物酶 (HRP)，并显示出增强的过氧化物酶样活性。因此，我们使用 PdCu TPs/PG 催化 HO 的电化学活性基质并产生协同放大的电流信号，用于随后对 HBeAg 的敏感检测。由于 PdCu TPs/PG 的良好导电性、大比表面积和协同放大作用，HBeAg 的定量检测显示出 20 fg·mL 的检测限和 60 fg·mL 至 100 ng·mL 的线性范围。在检测人血清样品时，基于标准添加法和与 ELISA 的比较，PdCu TPs/PG 表现出良好的准确性。所制备的免疫传感器表现出良好的选择性、稳定性和重现性，为确定乙型肝炎的预后提供了重要依据，并显示出在医学应用中的潜在应用。
