Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Departement of Chemistry, Faculty of Science, Brawijaya University, Malang 65145, Indonesia.
ScientificWorldJournal. 2021 Feb 26;2021:6643573. doi: 10.1155/2021/6643573. eCollection 2021.
Hepatitis B virus (HBV) still remains a major global public health problem. One-half to one-third of the total HBV infected people died due to late detection of HBV. Serological antigen and viral HBV detections can help in the diagnosis, referral, and treatment of HBV. Available methods for HBV detection mostly used bulky instruments. Miniaturization of devices for HBV detection has been started by narrowing down the size of the devices. Several methods have also been proposed to increase the selectivity and sensitivity of the miniaturized methods, such as sandwich recognition of the biomarkers and the use of nano- to micro-sized materials. This review presents recent HBV detections in the last two decades from laboratory-based instruments towards microfluidic paper-based analytical devices (PADs) for point-of-care testing (POCT) purposes. Early and routine analysis to detect HBV as early as possible could be achieved by POCT, especially for areas with limited access to a central laboratory and/or medical facilities.
乙型肝炎病毒（HBV）仍然是一个主要的全球公共卫生问题。由于 HBV 的检测不及时，总共有一半到三分之一的 HBV 感染者死亡。血清学抗原和病毒 HBV 的检测有助于 HBV 的诊断、转诊和治疗。HBV 的现有检测方法大多使用体积庞大的仪器。HBV 检测设备的小型化已经通过缩小设备的尺寸开始了。已经提出了几种方法来提高小型化方法的选择性和灵敏度，例如生物标志物的夹心识别和使用纳米到微尺寸的材料。本综述介绍了过去二十年中，从基于实验室的仪器到用于即时检测（POCT）目的的微流控纸基分析装置（PAD），对 HBV 的检测进展。通过 POCT 可以实现早期和常规分析，尽早检测 HBV，特别是对于那些难以获得中央实验室和/或医疗设施的地区。
