YAP基因缺失的诱导多能干细胞MUSIi012-A-2保持了所有正常干细胞特征。

YAP-depleted iPSC MUSIi012-A-2 maintained all normal stem cell characteristics.

作者信息

Lorthongpanich Chanchao, Laowtammathron Chuti, Jiamvoraphong Nittaya, Srisook Pimonwan, Chingsuwanrote Pimjai, Klaihmon Phatchanat, Damkham Nattaya, Terbto Papussorn, Waeteekul Supaporn, U-Pratya Yaowalak, Issaragrisil Surapol

机构信息

Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.

出版信息

Stem Cell Res. 2020 Mar;43:101723. doi: 10.1016/j.scr.2020.101723. Epub 2020 Jan 27.

DOI:10.1016/j.scr.2020.101723

PMID:32018207

Abstract

Yes-associated protein (YAP) is an important transcriptional coactivator in the Hippo signaling pathway. Using CRISPR/Cas9 technology, we established a stable YAP-knockdown (YAP-KD) induced pluripotent stem cell (iPSC) from the MUSIi012-A cell line. The YAP-KD iPSC MUSIi012-A-2 maintained the pluripotent phenotype, the ability to differentiate into all three embryonic germ layers, and it maintained the normal karyotype.

摘要

Yes相关蛋白（YAP）是Hippo信号通路中的一种重要转录共激活因子。我们利用CRISPR/Cas9技术，从MUSIi012-A细胞系建立了稳定的YAP敲低（YAP-KD）诱导多能干细胞（iPSC）。YAP-KD iPSC MUSIi012-A-2维持了多能表型、分化为所有三个胚胎胚层的能力，并维持了正常的核型。