Liu Yu-Kuo, Chen Wei-Chuan, Huang Yu-Ching, Chang Yu-Kaung, Chu I-Ming, Tsai Shen-Long, Wei Yu-Hong
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, No. 259, Wenhua 1st Rd., Guishan Dist., TaoYuan City 33302, Taiwan, ROC.
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, No. 135 Yuan-Tung Road, Chung-Li Dist., Taoyuan City 32003, Taiwan, ROC.
J Biosci Bioeng. 2017 Aug;124(2):184-188. doi: 10.1016/j.jbiosc.2017.02.018. Epub 2017 Mar 18.
The aim of this study was to use a modified bioreactor system for simultaneous saccharification of cellulose and bioethanol production. We tested Aspergillus niger and Trichoderma reesei for cellulose saccharification and Zymomonas mobilis for bioethanol production simultaneously in this modified bioreactor. The results showed that various carboxymethylcellulose (CMC) concentrations (10, 15, or 20 g/L) as a substrate for A. niger and T. reesei yielded bioethanol production of 0.51, 0.78, and 0.89 g/L and a CMC conversion rate of 10.2%, 10.7%, and 8.89%, respectively. These data suggested that at 10 g/L CMC as a substrate, the CMC conversion rate was higher than that at the other concentrations. When CMC concentration exceeded 15 g/L, bioethanol production was prolonged to 40 h. These results were attributed to the viscosity of CMC. This study also tested Napier grass (an agricultural byproduct) for bioethanol production. The results revealed bioethanol production and the theoretical Napier grass conversion rate were 0.38 g/L and 12.6%, respectively, for 13-h cultivation when the feeding concentration of Napier grass was 10 g/L. When Napier grass concentration was increased to 15 g/L, bioethanol production and the Napier grass conversion rate reached 0.51 g/L and 23%, respectively, after 14-h cultivation. Eventually, the experimental results indicated using agricultural waste for bioethanol production has been become a potential strategy.
本研究的目的是使用改良的生物反应器系统同时进行纤维素糖化和生物乙醇生产。我们在这个改良的生物反应器中同时测试了黑曲霉和里氏木霉用于纤维素糖化，以及运动发酵单胞菌用于生物乙醇生产。结果表明，作为黑曲霉和里氏木霉底物的不同羧甲基纤维素（CMC）浓度（10、15或20 g/L）分别产生了0.51、0.78和0.89 g/L的生物乙醇产量以及10.2%、10.7%和8.89%的CMC转化率。这些数据表明，以10 g/L CMC作为底物时，CMC转化率高于其他浓度。当CMC浓度超过15 g/L时，生物乙醇生产延长至40小时。这些结果归因于CMC的粘度。本研究还测试了象草（一种农业副产品）用于生物乙醇生产。结果显示，当象草进料浓度为10 g/L时，培养13小时的生物乙醇产量和理论象草转化率分别为0.38 g/L和12.6%。当象草浓度增加到15 g/L时，培养14小时后生物乙醇产量和象草转化率分别达到0.51 g/L和23%。最终，实验结果表明利用农业废弃物生产生物乙醇已成为一种潜在策略。
