Lagerkvist U, Akesson B, Brändén R
J Biol Chem. 1977 Feb 10;252(3):1002-6.
The shape of the time curve for the aminoacylation of tRNA has been investigated using five different amino acid:tRNA ligases. Four of these enzymes showed a lag in the time curve during the early phase of the first catalytic turnover of the enzyme. In each case, the lag period could be abolished by preincubating the ligase with amino acid, ATP, and Mg2+ under conditions known to give an aminoacyl adenylate-enzyme complex. With all five ligases the steady state rate of transfer from the preformed aminoacyl-adenylate complex to tRNA was approximately the same as that of the overall reaction.
tRNA连接酶研究了tRNA氨酰化的时间曲线形状。其中四种酶在酶的首次催化周转早期阶段的时间曲线中出现滞后。在每种情况下，通过在已知能形成氨酰腺苷酸-酶复合物的条件下将连接酶与氨基酸、ATP和Mg2+预孵育，可以消除滞后阶段。对于所有五种连接酶，从预先形成的氨酰腺苷酸复合物转移到tRNA的稳态速率与整个反应的速率大致相同。
