ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.
UMIB - Unidade Multidisciplinar de Investigação Biomédica, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.
Sci Rep. 2020 Feb 4;10(1):1771. doi: 10.1038/s41598-020-58678-0.
Immune cells resident in adipose tissue have important functions in local and systemic metabolic homeostasis. Nevertheless, these immune cell populations remain poorly characterized in bovines. Recently, we described diverse lymphocyte subpopulations in adipose tissue of Holstein-Friesian cows. Here, we aimed at characterising myeloid cell populations present in bovine adipose tissue using multicolour flow cytometry, cell sorting and histochemistry/immunohistochemistry. Macrophages, CD14CD11bMHC-IICD45 cells, were identified in mesenteric and subcutaneous adipose tissue, though at higher proportions in the latter. Mast cells, identified as SSC-ACD11bCD14MHC-IICH138ACD45 cells, were also observed in adipose tissue and found at higher proportions than macrophages in mesenteric adipose tissue. Neutrophils, presenting a CH138ACD11b phenotype, were also detected in mesenteric and subcutaneous adipose tissue, however, at much lower frequencies than in the blood. Our gating strategy allowed identification of eosinophils in blood but not in adipose tissue although being detected by morphological analysis at low frequencies in some animals. A population not expressing CD45 and with the CH138A CD11bMHC-II phenotype, was found abundant and present at higher proportions in mesenteric than subcutaneous adipose tissue. The work reported here may be useful for further studies addressing the function of the described cells.
脂肪组织中驻留的免疫细胞在局部和全身代谢稳态中具有重要功能。然而，这些免疫细胞群体在牛中仍然描述不足。最近，我们描述了荷斯坦-弗里森奶牛脂肪组织中的多种淋巴细胞亚群。在这里，我们旨在使用多色流式细胞术、细胞分选和组织化学/免疫组织化学来描述牛脂肪组织中存在的髓样细胞群体。在肠系膜和皮下脂肪组织中鉴定出巨噬细胞、CD14+CD11b+MHC-II+CD45+细胞，但在后者中的比例更高。肥大细胞，鉴定为 SSC-ACD11b+CD14+MHC-II+H138+ACD45+细胞，也存在于脂肪组织中，在肠系膜脂肪组织中的比例高于巨噬细胞。中性粒细胞，表现出 CH138+CD11b+表型，也存在于肠系膜和皮下脂肪组织中，但在血液中的频率要低得多。我们的门控策略允许在血液中识别嗜酸性粒细胞，但不在脂肪组织中识别，尽管在一些动物中通过形态分析以低频率检测到。在血液中检测到但在脂肪组织中未检测到的不表达 CD45 且具有 CH138A CD11b+MHC-II 表型的细胞群体在肠系膜脂肪组织中丰富且比例高于皮下脂肪组织。本研究报告的工作可能有助于进一步研究描述细胞的功能。
