Teixeira Luzia, Correia Alexandra, Oliveira Bárbara M, Pinto Ana, Ferreira Paula G, Vilanova Manuel
ICBAS-Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
UMIB-Unidade Multidisciplinar de Investigação Biomédica, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
Pathogens. 2020 Jul 17;9(7):587. doi: 10.3390/pathogens9070587.
is an apicomplexan parasite that in cattle assumes particular importance, as it is responsible for abortions reported worldwide. Leptin is an adipokine mainly secreted by adipocytes, which beside its role in maintaining metabolic homeostasis also has important effects in both innate and adaptive immunity. In previous work, we showed that mice chronically infected with had elevated serum leptin levels. Here, we sought to assess whether acute infection with infection influenced the production of this adipokine as well as leptin receptor mRNA levels. Our results show that acute infection with led to decreased leptin serum levels and mRNA expression in adipose tissue. A decrease in leptin receptor transcript variant 1 mRNA (long isoform) and leptin receptor transcript variant 3 mRNA (one of the short isoforms) expression was also observed. An increase in the number of cells staining positive for leptin in the liver of infected mice was observed, although this increase was less marked in Interleukin (IL)-12/IL-23 p40-deficient mice. Overall, our results show that infection also influences leptin production during acute infection.
是一种顶复门寄生虫，在牛身上具有特别重要的意义，因为它是全球范围内报告的流产原因。瘦素是一种主要由脂肪细胞分泌的脂肪因子，除了在维持代谢稳态方面的作用外，在先天免疫和适应性免疫中也具有重要作用。在先前的工作中，我们表明长期感染的小鼠血清瘦素水平升高。在这里，我们试图评估急性感染是否会影响这种脂肪因子的产生以及瘦素受体mRNA水平。我们的结果表明，急性感染导致脂肪组织中瘦素血清水平和mRNA表达降低。还观察到瘦素受体转录变体1 mRNA（长异构体）和瘦素受体转录变体3 mRNA（短异构体之一）表达减少。在感染小鼠的肝脏中，观察到瘦素染色阳性细胞数量增加，尽管在白细胞介素（IL）-12/IL-23 p40缺陷小鼠中这种增加不太明显。总体而言，我们的结果表明，感染在急性感染期间也会影响瘦素的产生。
