ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.
UMIB -Unidade Multidisciplinar de Investigação Biomédica, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.
Sci Rep. 2019 Mar 4;9(1):3413. doi: 10.1038/s41598-019-39938-0.
The importance of immune cells present in the adipose tissue to metabolic homeostasis has been increasingly recognized. Nevertheless, in bovines few studies have so far addressed the immune cell populations resident in this tissue. Here we developed an eight-colour flow cytometry panel to address T cell populations present in bovine adipose tissue. Our results showed that γδ T cells, CD4 and CD8 CD3 non-γδ T cells, as well as NK cells, are present in the mesenteric and subcutaneous adipose tissue of Holstein-Friesian cows. The frequency of both γδ T cells and CD8 non-γδ T cells was found higher in mesenteric than in subcutaneous adipose tissue. The majority of T cells in adipose tissue presented a CD45ROCD62L phenotype, characteristic of effector memory cells, and the frequency of these cellular populations was higher than in the blood. The ratio of CD4 T cells over CD8 T cells was similar between subcutaneous and mesenteric adipose tissue but different from the one found in blood. Overall, our results highlight particular phenotypic characteristics of bovine adipose tissue T cell populations.
脂肪组织中存在的免疫细胞对代谢稳态的重要性已逐渐被认识。然而，到目前为止，在牛中，很少有研究涉及到存在于该组织中的免疫细胞群体。在这里，我们开发了一个八色流式细胞术面板来研究牛脂肪组织中存在的 T 细胞群体。我们的结果表明，γδ T 细胞、CD4 和 CD8 CD3 非 γδ T 细胞以及 NK 细胞存在于荷斯坦-弗里森奶牛的肠系膜和皮下脂肪组织中。γδ T 细胞和 CD8 非 γδ T 细胞在肠系膜脂肪组织中的频率均高于皮下脂肪组织。脂肪组织中的大多数 T 细胞表现出 CD45ROCD62L 表型，这是效应记忆细胞的特征，这些细胞群体的频率高于血液。皮下和肠系膜脂肪组织中 CD4 T 细胞与 CD8 T 细胞的比值与血液中的比值相似，但与血液中的比值不同。总的来说，我们的结果突出了牛脂肪组织 T 细胞群体的特定表型特征。
