Dermon C R, Kouvelas E D
Department of Physiology, School of Medicine, University of Patras, Greece.
Int J Dev Neurosci. 1988;6(5):471-82. doi: 10.1016/0736-5748(88)90053-6.
The pharmacological properties of [3H]-WB4101, [3H]-clonidine and [3H]-dihydroalprenolol binding in chick brain membranes display the characteristics known for alpha 1-, alpha 2- and beta-adrenergic binding sites, respectively. Kinetic studies performed at different embryonic and post-hatching ages have shown one binding component for each one of the above radioactive ligands. The ontogeny of alpha 1, alpha 2 and beta binding sites was studied in cerebral hemispheres, optic lobes, brain stem and cerebellum. In all brain regions studied, the development of alpha 2 binding sites precedes that of alpha 1 and beta, and a very significant decrease of alpha 2 number was observed in the cerebellum, brain stem and optic lobes afterwards. The autoradiographic localization of adrenergic receptors was studied in the optic lobes and cerebellum. In the optic lobes the superficial layers of stratum griseum and fibrosum showed a strong selective labelling of alpha 1, alpha 2 and beta binding sites and the strong selective labelling of alpha 2 binding sites extended to the layer of stratum opticum. Among the nuclei located in the optic lobe only the nucleus mesencephalis lateralis pars dorsalis (MLD) exhibited a strong selective labelling for alpha 1 binding sites while, for beta binding sites, not only the MLD, but also the nucleus isthmic pars parvocellularis (Ipc) and the nucleus isthmic pars magnocellularisa (Imc) exhibited strong labelling. In the cerebellum strong selective labelling for alpha 1 and beta receptors was seen in the molecular layer. Labelling of the granule cell layer was almost equally strong for alpha 1 but significantly less for beta binding sites. No significant labelling could be detected for alpha 2 binding sites.
[3H]-WB4101、[3H]-可乐定和[3H]-二氢丙萘洛尔在鸡脑膜中的结合所表现出的药理学特性，分别显示出已知的α1、α2和β肾上腺素能结合位点的特征。在不同胚胎期和孵化后阶段进行的动力学研究表明，上述每种放射性配体均有一个结合成分。对大脑半球、视叶、脑干和小脑的α1、α2和β结合位点的个体发生进行了研究。在所有研究的脑区中，α2结合位点的发育先于α1和β结合位点，随后在小脑、脑干和视叶中观察到α2结合位点数量显著减少。对视叶和小脑中肾上腺素能受体进行了放射自显影定位研究。在视叶中，灰质和纤维层的表层对α1、α2和β结合位点有强烈的选择性标记，α2结合位点的强烈选择性标记延伸至视层。在视叶中的核团中，只有中脑外侧背侧核（MLD）对α1结合位点有强烈的选择性标记，而对于β结合位点，不仅MLD，而且峡部小细胞部核（Ipc）和峡部大细胞部核（Imc）都有强烈标记。在小脑中，分子层对α1和β受体有强烈的选择性标记。颗粒细胞层对α1的标记几乎同样强烈，但对β结合位点的标记明显较弱。未检测到α2结合位点的明显标记。
