Plant Breeding and Acclimatization Institute - National Research Institute, Młochów Research Center, Platanowa 19, 05-831, Młochów, Poland.
Mol Biol Rep. 2020 Mar;47(3):1713-1722. doi: 10.1007/s11033-020-05263-3. Epub 2020 Feb 4.
A conversion of amyloplasts into chloroplasts in the potato tuber after light exposure is known as tuber greening and is one of the major causes of tuber loss. We report here the first mapping of the factors affecting tuber greening in potato. We used an F1 mapping population of diploid potatoes and DArTseq™ markers to construct a genetic map. The individuals of the mapping population, parents and standards were phenotyped for two tuber greening parameters: external tuber greening and internal greening depth on 0-5 scales in three years 2015, 2016 and 2018. The results were used for the analysis of Quantitative Trait Loci (QTLs) by an interval QTL mapping. Two most important QTLs were covering large regions of chromosomes VII and X and had the strongest effect on both greening parameters in data sets obtained in particular years and in the mean data set. Variance observed in the mean tuber greening could be ascribed in 16.9% to the QTL on chromosome VII and in 23.4% to the QTL on chromosome X. The QTL on chromosome VII explained 13.1%, while the QTL on chromosome X explained up to 17.7% of the variance in the mean tuber greening depth. Additional, minor QTLs were year- and/or trait-specific. The QTLs on chromosomes VII and X determine big parts of the observed tuber greening variation and should be investigated further in order to identify the genes underlying their effects but also should be taken into account when selecting non-greening potato lines in the breeding process.
在光照下，马铃薯块茎中的淀粉体转化为叶绿体，这一过程被称为块茎绿变，是块茎损失的主要原因之一。我们在此报告了首次对影响马铃薯块茎绿变的因素进行的定位。我们使用二倍体马铃薯的 F1 作图群体和 DArTseqTM 标记构建了遗传图谱。在 2015、2016 和 2018 年的三年中，对作图群体、亲本和标准品的两个块茎绿变参数（外部块茎绿变和内部绿变深度）进行了表型分析，评分范围为 0-5 分。结果用于通过区间 QTL 作图分析数量性状位点（QTL）。两个最重要的 QTL 覆盖了染色体 VII 和 X 的大片段，对特定年份和平均值数据集的两个绿变参数都有最强的影响。在平均块茎绿变中观察到的方差可以归因于染色体 VII 上的 QTL 为 16.9%，归因于染色体 X 上的 QTL 为 23.4%。染色体 VII 上的 QTL 解释了 13.1%，而染色体 X 上的 QTL 解释了平均块茎绿变深度方差的 17.7%。此外，还有次要的 QTL 具有年份和/或性状特异性。染色体 VII 和 X 上的 QTL 决定了观察到的块茎绿变变化的大部分，应该进一步研究以鉴定其效应背后的基因，但在选育非绿变马铃薯品系时也应该考虑到这些 QTL。
