Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
National Chemistry Experimental Teaching Demonstration Center, Jilin University, Changchun, 130012, China.
J Fluoresc. 2020 Mar;30(2):329-334. doi: 10.1007/s10895-020-02502-5. Epub 2020 Feb 4.
Excessive secretion of PTH leads to disturbance of calcium and phosphorus metabolism in the body, which promotes bone, kidney, digestive system and nervous system diseases. Due to the short half-life of PTH, it becomes a difficult issue for PTH detection in the clinical diagnosis field. We explored a competitive immunofluorescent sensing mode based on FRET of two-color CdTe QDs for ratiometric PTH 1-84 antigen detection. The FRET effect and ratiometric fluorescence between the two-color CdTe QDs motivated accurate quantification of PTH 1-84 antigen concentration from 0.01 ng mL to 0.08 ng mL with a limit of detection of 3 pg mL. More importantly, under UV irradiation, samples with different concentrations of PTH 1-84 antigen achieved fluorescence visualization, which provides huge possibility for the practical application of PTH 1-84 antigen point-of-care detection.
甲状旁腺素的过度分泌会导致体内钙磷代谢紊乱，从而促进骨骼、肾脏、消化系统和神经系统疾病的发生。由于甲状旁腺素的半衰期较短，因此在临床诊断领域进行甲状旁腺素检测成为一个难题。我们探索了一种基于双色 CdTe QD 的 FRET 的竞争免疫荧光传感模式，用于甲状旁腺素 1-84 抗原的比率检测。双色 CdTe QD 的 FRET 效应和比率荧光可实现对 PTH 1-84 抗原浓度从 0.01ng/mL 到 0.08ng/mL 的精确定量，检测限低至 3pg/mL。更重要的是，在紫外光照射下，不同浓度的 PTH 1-84 抗原样本实现了荧光可视化，这为甲状旁腺素 1-84 抗原即时检测的实际应用提供了巨大的可能性。
