Department of Horticulture, School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
Department of Food Science, School of Food Science and Engineering, Yangzhou University, Yangzhou, China.
Plant Cell Environ. 2020 Jun;43(6):1545-1557. doi: 10.1111/pce.13738. Epub 2020 Feb 18.
Waterlogging is a severe environmental stress that causes severe crop productivity losses. Cucumber (Cucumis sativus L.) survives waterlogging by producing adventitious roots (ARs) that enhance gas exchange. Little is known about the role of light and sugars in the waterlogging-induced production of ARs. The role of these factors in AR production was therefore studied in cucumber seedlings grown in the absence or presence of waterlogging and different light conditions. The effect of photosynthesis was studied by removing the shoots of the seedlings and replacing them with exogenous applications of sucrose or stachyose. Shoot removal inhibited AR emergence and elongation. However, the exogenous application of sugars fully restored AR emergence and partially restored root elongation. The exogenous application of a synthetic auxin restored AR emergence but not AR elongation. Transcriptome profiling analysis was used to determine the effects of light on gene expression in the hypocotyls under these conditions. The levels of transcripts encoding proteins involved in auxin transport and signalling were higher in the light and following the exogenous application of sucrose and stachyose. These results show that the waterlogging-induced emergence of ARs is regulated by the interaction between sugars and auxin, whereas AR elongation depends only on sugars alone.
涝渍是一种严重的环境胁迫，会导致严重的作物生产力损失。黄瓜（Cucumis sativus L.）通过产生不定根（AR）来适应涝渍，从而增强气体交换。关于光照和糖在涝渍诱导产生 AR 中的作用知之甚少。因此，本研究在无涝渍和有涝渍以及不同光照条件下生长的黄瓜幼苗中研究了这些因素在 AR 产生中的作用。通过去除幼苗的地上部分并用外源性蔗糖或棉子糖替代来研究光合作用的作用。去除地上部分抑制了不定根的出现和伸长。然而，糖的外源应用完全恢复了不定根的出现，并部分恢复了根的伸长。合成生长素的外源应用恢复了不定根的出现，但不能恢复不定根的伸长。转录组谱分析用于确定在这些条件下光照对下胚轴基因表达的影响。在光照下以及外源蔗糖和棉子糖处理后，编码参与生长素运输和信号转导的蛋白质的转录本水平较高。这些结果表明，不定根的涝渍诱导出现受糖和生长素之间的相互作用调节，而不定根的伸长仅取决于糖。
