Wu Hongying, Yu Haitao, Zhang Xingen, Wang Yixiao, Zhu Hongjia, Zhao Yang, Ma Qing
Anhui Agricultural University, School of Life Sciences, National Engineering Laboratory of Crop Stress Resistance Breeding, Hefei, China.
West Anhui University, College of Biological and Pharmaceutical Engineering, Lu'an, China.
Genet Mol Biol. 2024 Jan 8;46(4):e20230026. doi: 10.1590/1678-4685-GMB-2023-0026. eCollection 2024.
Waterlogging stress is an important abiotic stress that adversely affects maize growth and yield. The mechanism regulating the early stage of the maize response to waterlogging stress is largely unknown. In this study, CM37 and cmh15 seedlings were treated with waterlogging stress and then examined in terms of their physiological changes. The results indicated that inbred line cmh15 is more tolerant to waterlogging stress and less susceptible to peroxide-based damages than CM37. The RNA sequencing analysis identified 1,359 down-regulated genes and 830 up-regulated genes in the waterlogging-treated cmh15 plants (relative to the corresponding control levels). According to the Gene Ontology analysis for the differentially expressed genes (DEGs), some important terms were identified which may play important roles in the response to waterlogging stress. Moreover, enriched Kyoto Encyclopedia of Genes and Genomes pathways were also identified for the DEGs. Furthermore, the substantial changes in the expression of 36 key transcription factors may be closely related to the maize in response to waterlogging stress. This study offers important insights into the mechanism in regulating maize tolerance to waterlogging stress, with important foundations for future research.
涝渍胁迫是一种重要的非生物胁迫，对玉米生长和产量产生不利影响。玉米对涝渍胁迫早期响应的调控机制在很大程度上尚不清楚。在本研究中，对CM37和cmh15幼苗进行涝渍胁迫处理，然后检测其生理变化。结果表明，自交系cmh15比CM37更耐涝渍胁迫，对基于过氧化物的损伤更不敏感。RNA测序分析确定了涝渍处理的cmh15植株中有1359个下调基因和830个上调基因（相对于相应的对照水平）。根据对差异表达基因（DEGs）的基因本体分析，确定了一些可能在对涝渍胁迫的响应中起重要作用的重要术语。此外，还为DEGs确定了富集的京都基因与基因组百科全书途径。此外，36个关键转录因子表达的显著变化可能与玉米对涝渍胁迫的响应密切相关。本研究为调控玉米耐涝渍胁迫的机制提供了重要见解，为未来的研究奠定了重要基础。
