Department of Analytical Chemistry, Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas-Unicamp, Campinas, SP, Brazil.
National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas-Unicamp, Campinas, SP, Brazil.
Rapid Commun Mass Spectrom. 2020 Sep;34 Suppl 3:e8726. doi: 10.1002/rcm.8726. Epub 2020 Feb 28.
The widespread application of nanoparticles (NPs) in recent times has caused concern because of their effects in biological systems. Although NPs can be produced naturally, industrially synthesized NPs affect the metabolism of a given organism because of their high reactivity. The biotransformation of NPs involves different processes, including aggregation/agglomeration, and reactions with biomolecules that will be reflected in their toxicity. Several analytical techniques, including inductively coupled plasma mass spectrometry (ICP-MS), have been used for characterizing and quantifying NPs in biological samples. In fact, in addition to providing information regarding the morphology and concentration of NPs, ICP-MS-based platforms, such as liquid chromatography/ICP-MS, single-particle ICP-MS, field-flow fractionation (asymmetrical flow field-flow fractionation)-ICP-MS, and laser ablation-ICP-MS, yield elemental information about molecules. Furthermore, such information together with speciation analysis enlarges our understanding of the interaction between NPs and biological organisms. This study reports the contribution of ICP-MS-based platforms as a tool for evaluating NPs in distinct biological samples by providing an additional understanding of the behavior of NPs and their toxicity in these organisms.
近年来，由于纳米粒子（NPs）在生物系统中的作用，它们的广泛应用引起了人们的关注。尽管 NPs 可以自然产生，但由于其高反应性，工业合成的 NPs 会影响给定生物体的新陈代谢。NPs 的生物转化涉及不同的过程，包括聚集/团聚，以及与生物分子的反应，这将反映在它们的毒性中。几种分析技术，包括电感耦合等离子体质谱（ICP-MS），已被用于生物样品中 NPs 的表征和定量。事实上，除了提供有关 NPs 形态和浓度的信息外，基于 ICP-MS 的平台，如液相色谱/ICP-MS、单颗粒 ICP-MS、场流分离（不对称流场流分离）-ICP-MS 和激光烧蚀-ICP-MS，还可以提供有关分子的元素信息。此外，这些信息与形态分析一起，扩大了我们对 NPs 与生物有机体相互作用的理解。本研究报告了基于 ICP-MS 的平台作为评估不同生物样品中 NPs 的工具的贡献，通过提供对 NPs 行为及其在这些生物体中的毒性的更深入理解。
