鸡肌腱异种移植中细胞接种的成功率和效率——肌腱和韧带重建的一种有前景的替代方法。
Success and efficiency of cell seeding in Avian Tendon Xenografts - A promising alternative for tendon and ligament reconstruction.
作者信息
Thönnes Simon, Shelton Peter, Bracey Daniel N, Van Dyke Mark, Whitlock Patrick, Smith Thomas L, Moghaddam Arash, Tuohy Christopher
机构信息
Department of Orthopaedic Surgery, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA.
HTRG - Heidelberg Trauma Research Group, Division of Trauma and Reconstructive Surgery, Center for Orthopaedics, Trauma Surgery and Spinal Cord Injury, University Hospital Heidelberg, Schlierbacher Landstr. 200a, D-69118, Heidelberg, Germany.
出版信息
J Orthop. 2019 Sep 12;18:155-161. doi: 10.1016/j.jor.2019.09.010. eCollection 2020 Mar-Apr.
Decellularized tendon xenografts offer a promising alternative for reconstruction by using ubiquitously available material. This study compares static and centrifugal seeding of avian tendon scaffolds with NIH 3T3 fibroblasts. Incorporation of viable cells was achievable with both techniques, represented by DNA content. Proliferation rate and viability assay showed neither damage by centrifugal force nor superiority of the technique. Cell proliferation after 10 days of culture demonstrated that the scaffold did not hinder 3-D culturing. Confocal laser microscopy revealed structural details as formation of focal adhesions, to provide deeper insight into the process of cell attachment and growth in xenografts.
去细胞化肌腱异种移植物为使用随处可得的材料进行重建提供了一种有前景的替代方案。本研究比较了用NIH 3T3成纤维细胞对禽类肌腱支架进行静态接种和离心接种的效果。两种技术均能实现活细胞的掺入，以DNA含量为代表。增殖率和活力测定表明，离心力既没有造成损伤，该技术也没有优势。培养10天后的细胞增殖表明，支架不妨碍三维培养。共聚焦激光显微镜揭示了诸如粘着斑形成等结构细节，以便更深入地了解异种移植物中细胞附着和生长的过程。
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