Formaker B K, Hill D L
Department of Psychology, University of Virginia, Charlottesville 22903.
Am J Physiol. 1988 Dec;255(6 Pt 2):R1002-7. doi: 10.1152/ajpregu.1988.255.6.R1002.
The identity of the residual taste response to NaCl after lingual application of the sodium transport blocker, amiloride, was studied by electrophysiological recordings from the rat chorda tympani nerve. Stimulation of the anterior tongue with salt solutions resulted in responses to halogenated sodium salts that were not eliminated by amiloride; approximately 30% of the halogenated sodium salt response remained after amiloride. In contrast, responses to nonhalogenated sodium salts were reduced to less than 4% of the original response after amiloride. To further learn of characteristics relating to the residual NaCl response, binary mixture and cross-adaptation experiments were accomplished. Responses to mixtures of sodium acetate or sodium bicarbonate with choline chloride were similar to responses elicited by equimolar concentrations of NaCl before and after amiloride. Moreover, NaCl and NaBr cross-adapted with choline chloride after, but not before, lingual application of amiloride. These experiments indicate that the residual response to halogenated sodium salts (e.g., NaCl) is related to the halogen itself, and from findings presented here, we propose one pathway for sodium taste transduction.
通过对大鼠鼓索神经进行电生理记录，研究了在舌部应用钠转运阻滞剂氨氯吡咪后对氯化钠残留味觉反应的特性。用盐溶液刺激舌尖会引发对卤化钠盐的反应，而这种反应不会被氨氯吡咪消除；氨氯吡咪处理后，约30%的卤化钠盐反应仍然存在。相比之下，氨氯吡咪处理后对非卤化钠盐的反应降低至原始反应的4%以下。为了进一步了解与残留氯化钠反应相关的特性，进行了二元混合物和交叉适应实验。对醋酸钠或碳酸氢钠与氯化胆碱混合物的反应，在氨氯吡咪处理前后与等摩尔浓度氯化钠引发的反应相似。此外，在舌部应用氨氯吡咪后，氯化钠和溴化钠与氯化胆碱发生交叉适应，但在此之前不会。这些实验表明，对卤化钠盐（如氯化钠）的残留反应与卤素本身有关，基于此处呈现的研究结果，我们提出了一种钠味觉转导途径。
