Faculty of Food Science and Engineering, "Dunarea de Jos" University of Galaţi, Galaţi, Romania.
Faculty of Medicine and Pharmacy, "Dunarea de Jos" University of Galaţi, Galaţi, Romania.
J Environ Sci Health B. 2020;55(5):484-494. doi: 10.1080/03601234.2020.1724016. Epub 2020 Feb 5.
Given that the pesticide and fungicide residues determination in honey is not a routine analysis in Romania, information on these emerging contaminants is useful for consumer's safety. High resolution mass spectrometry technique was applied by Q-Exactive Orbitrap LC-MS/MS to identify and quantify environmental contaminants in honey. A list of 25 compounds, biocides and antifungals was selected for the method development, based on the occurrence in the Romanian environment and their potential usage in agriculture. The method was applied for 18 various honey samples collected in different geographic regions of Romania. Eleven compounds were present in the honey samples: carbendazim, enilconazole, hexaconazole, penconazole, tebuconazole, flusilazole, thiabendazole, terconazole, cyproconazole, propiconazole, metalaxyl. Targeted MS/MS analyses were performed for confirmation. The measured quantities ranged from 1.7-7.2 μg kg, lower than MRLs established by the legislation. The most abundant compound was enilconazole (imazalil), which was detected in fourteen samples. To the best of our knowledge, the present study is the first one concerning antifungal contamination of honey in Romania. The results proved that the tested honey samples are safe for human consumption.
鉴于罗马尼亚对蜂蜜中的农药和杀菌剂残留的检测并非常规分析，因此有关这些新兴污染物的信息对消费者的安全很有用。高分辨质谱技术通过 Q-Exactive Orbitrap LC-MS/MS 用于鉴定和定量蜂蜜中的环境污染物。根据在罗马尼亚环境中的出现情况及其在农业中的潜在用途，选择了 25 种化合物（杀生物剂和杀真菌剂）来开发该方法。该方法应用于在罗马尼亚不同地理区域采集的 18 种不同的蜂蜜样品。在蜂蜜样品中存在 11 种化合物：多菌灵、烯唑醇、己唑醇、丙环唑、戊唑醇、氟硅唑、噻菌灵、三唑酮、环丙唑醇、丙环唑、甲霜灵。进行了靶向 MS/MS 分析以确认。测量的数量范围为 1.7-7.2μg kg，低于法规规定的 MRLs。最丰富的化合物是烯唑醇（咪鲜胺），在 14 个样品中均有检测到。据我们所知，本研究是关于罗马尼亚蜂蜜中抗真菌污染的首次研究。结果证明，所测试的蜂蜜样品可安全供人类食用。
