Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331.
Department of Plant Sciences, University of California-Davis, Davis, CA 95616.
Phytopathology. 2020 May;110(5):1082-1092. doi: 10.1094/PHYTO-11-19-0415-R. Epub 2020 Apr 6.
Stripe rust (incited by f. sp. ) and stem rust (incited by f. sp. ) are two of the most important diseases affecting barley. Building on prior work involving the introgression of the resistance genes into diverse genetic backgrounds and the discovery of additional quantitative trait locus (QTLs) for stem rust resistance, we generated an array of germplasm in which we mapped resistance to stripe rust and stem rust. Stem rust races TTKSK and QCCJB were used for resistance mapping at the seedling and adult plant stages, respectively. Resistance to stripe rust, at the adult plant stage, was determined by QTLs on chromosomes 1H, 4H, and 5H that were previously reported in the literature. The complex was validated as a source of resistance to stem rust at the seedling stage. Some parental germplasm, selected as potentially resistant to stem rust or susceptible but having other positive attributes, showed resistance at the seedling stage, which appears to be allelic to . The complex, and this new allele, were not sufficient for adult plant resistance to stem rust in one environment. A QTL on 5H, distinct from and a previously reported resistance QTL, was required for resistance at the adult plant stage in all environments. This QTL is coincident with the QTL for stripe rust resistance. Germplasm with mapped genes/QTLs conferring resistance to stripe and stem rust was identified and is available as a resource to the research and breeding communities.
条锈病（由 f. sp. 引起）和秆锈病（由 f. sp. 引起）是影响大麦的两种最重要的疾病。基于先前涉及将抗性基因 导入不同遗传背景的工作以及发现额外的秆锈病抗性数量性状位点（QTL），我们生成了一系列种质资源，我们在其中对条锈病和秆锈病进行了抗性作图。使用 TTKSK 和 QCCJB 两种秆锈菌系分别在幼苗和成株阶段进行抗性作图。成株期的条锈病抗性由先前文献中报道的 1H、4H 和 5H 染色体上的 QTL 决定。 复合体被验证为幼苗期秆锈病抗性的来源。一些亲本种质资源，被选择为可能对秆锈病具有抗性或易感但具有其他积极属性，在幼苗期表现出抗性，这似乎与 等位。 复合体和这个新的等位基因在一个环境中不足以使成株期对秆锈病具有抗性。在所有环境中，在 5H 上的一个不同于 和先前报道的抗性 QTL 的 QTL 是成株期抗性所必需的。这个 QTL 与条锈病抗性的 QTL 一致。带有条锈病和秆锈病抗性基因/QTL 的种质资源已被鉴定出来，并作为研究和育种社区的资源提供。
