Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, Puławy, Poland.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2020 Apr;37(4):622-633. doi: 10.1080/19440049.2020.1719284. Epub 2020 Feb 5.
Tea samples from the Polish market were analysed for the determination of pyrrolizidine alkaloids (PAs). The method applied in the study is based on the GC-MS technique and uses the sum parameter approach for quantification. The analytical protocol was subjected to validation in terms of linearity, repeatability, reproducibility, the limit of quantification and determination, matrix effect, selectivity, and uncertainty, and all evaluated parameters fulfilled the requirements of Commission Decision 2002/657. A total of 78% of the analysed samples contained 1,2-unsaturated PAs, and the concentrations were determined to fall in the range of 2-6498 µg kg. The highly contaminated samples were additionally subjected to LC-MS analysis. Much higher PA concentrations were determined with the sum parameter method, proving its superior quantification capability. The high contamination found prompted an assessment of the dietary exposure from the consumption of infusions prepared from the analysed teas. Two approaches were adopted: one assuming 100% transfer of PAs to the infusions and the second assuming only partial transfer. Regardless of the adopted approach, consumption of several infusions was shown to greatly exceed the maximum acceptable daily intake of PAs.
对波兰市场上的茶叶样品进行了分析，以测定吡咯里西啶生物碱（PAs）。本研究中应用的方法基于 GC-MS 技术，并采用总和参数法进行定量。分析方案在线性、重复性、再现性、定量和测定限、基质效应、选择性和不确定性方面进行了验证，所有评估参数均符合委员会第 2002/657 号决定的要求。分析的样品中有 78%含有 1,2-不饱和 PAs，其浓度范围为 2-6498µg kg。高度污染的样品还进行了 LC-MS 分析。总和参数法测定的 PA 浓度要高得多，证明其具有优越的定量能力。高污染情况促使对从分析的茶中冲泡的茶饮料的饮食暴露进行评估。采用了两种方法：一种方法假设 PAs 100%转移到冲泡液中，另一种方法假设仅部分转移。无论采用哪种方法，饮用几种冲泡液都被证明大大超过了 PAs 的最大可接受日摄入量。
