College of Agronomy, National Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou, China.
National Maize Improvement Center, China Agricultural University, Beijing, China.
PLoS One. 2020 Feb 5;15(2):e0228411. doi: 10.1371/journal.pone.0228411. eCollection 2020.
Kernel abortion is common phenomenon in vivo haploid induction and closely linked with haploid induction rate, but little information of kernel abortion is available and its genetic basis still unclear. We used two mapping populations including 186 and 263 F2.3 family lines to analyze the different degree of kernel abortion and identify quantitative trait loci (QTL) responsible for kernel abortion during haploid induction. In total 62 putative QTL, accounting for 3.27-14.70% of the phenotypic variation in kernel abortion traits, were detected across all 10 chromosomes. Ten QTL with over 10% contribution to phenotypic variation were affecting the fifth level of endosperm abortion (EnA5th), endosperm abortion (EnA) and total abortion (TA). Co-localization among kernel abortion traits QTL was observed in both populations and among different kernel abortion types. Five overlaps were indentified in the QTL for kernel abortion traits and HIR traits. Maize chromosome bins 3.01-3.02, 3.04-3.06, 4.05-4.06, 5.03-5.04, 8.06 were QTL hotspots for three or four traits related to the kernel abortion during haploid induction. Total kernel abortion rate (TAR) and HIR showed highly significant positive correlation. These findings may help to reveal haploid induction mechanisms and improve haploid production efficiency.
核败育是活体诱导单倍体过程中的常见现象，与单倍体诱导率密切相关，但有关核败育的信息很少，其遗传基础仍不清楚。我们利用包括 186 和 263 个 F2.3 家系的两个作图群体，分析了不同程度的核败育，并鉴定了在单倍体诱导过程中导致核败育的数量性状基因座（QTL）。总共检测到 62 个假定 QTL，占核败育性状表型变异的 3.27-14.70%，分布在所有 10 条染色体上。10 个对表型变异有超过 10%贡献的 QTL 影响了第五级胚乳败育（EnA5th）、胚乳败育（EnA）和总败育（TA）。在两个群体和不同的核败育类型中都观察到了核败育性状 QTL 之间的共定位。在核败育性状和 HIR 性状的 QTL 中鉴定出了 5 个重叠。玉米染色体 bin3.01-3.02、3.04-3.06、4.05-4.06、5.03-5.04、8.06 是与单倍体诱导过程中核败育相关的三个或四个性状的 QTL 热点。总核败育率（TAR）和 HIR 表现出高度显著的正相关。这些发现可能有助于揭示单倍体诱导机制，提高单倍体生产效率。
