Emory Transplant Center, Emory University School of Medicine, Atlanta, GA, USA; Department of Pathology & Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA.
Emory Transplant Center, Emory University School of Medicine, Atlanta, GA, USA.
Cell Rep. 2020 Feb 4;30(5):1282-1291.e5. doi: 10.1016/j.celrep.2020.01.016.
The identity of CD45 isoforms on the T cell surface changes following the activation of naive T cells and impacts intracellular signaling. In this study, we find that the anti-viral memory CD8 T pool is unexpectedly comprised of both CD45RB and CD45RB populations. Relative to CD45RB memory T cells, CD45RB memory T cells have lower affinity and display greater clonal diversity, as well as a persistent CD27 phenotype. The CD45RB memory population displays a homeostatic survival advantage in vivo relative to CD45RB memory, and long-lived high-affinity cells that persisted long term convert from CD45RB to CD45RB. Human CD45RO memory is comprised of both CD45RB and CD45RB populations with distinct phenotypes, and antigen-specific memory to two viruses is predominantly CD45RB. These data demonstrate that CD45RB status is distinct from the conventional central/effector T cell memory classification and has potential utility for monitoring and characterizing pathogen-specific CD8 T cell responses.
T 细胞表面的 CD45 同工型在幼稚 T 细胞激活后发生变化，并影响细胞内信号转导。在这项研究中，我们发现抗病毒记忆 CD8 T 细胞池出乎意料地由 CD45RB 和 CD45RB 群体组成。与 CD45RB 记忆 T 细胞相比，CD45RB 记忆 T 细胞具有较低的亲和力，表现出更大的克隆多样性以及持续的 CD27 表型。与 CD45RB 记忆细胞相比，CD45RB 记忆细胞在体内具有更好的稳态存活优势，并且长期存在的高亲和力细胞从 CD45RB 转换为 CD45RB。人类 CD45RO 记忆细胞由具有不同表型的 CD45RB 和 CD45RB 群体组成，对两种病毒的抗原特异性记忆主要是 CD45RB。这些数据表明，CD45RB 状态与传统的中央/效应 T 细胞记忆分类不同，并且具有监测和表征病原体特异性 CD8 T 细胞反应的潜在用途。
