Moreno Ricardo, Nájera Laura, Mascaraque Marta, Juarranz Ángeles, González Salvador, Gilaberte Yolanda
Dermatology Service, Hospital Univ. del Henares, Coslada, 28822 Madrid, Spain.
Pathology Service, Hospital Puerta de Hierro, Majadahonda, 28222 Madrid, Spain.
J Clin Med. 2020 Feb 1;9(2):398. doi: 10.3390/jcm9020398.
In mouse models of squamous cell carcinoma, pre-treatment with calcitriol prior to photodynamic therapy with aminolevulinic acid (ALA) enhances tumor cell death. We have evaluated the association between vitamin D status and the response of actinic keratoses to photodynamic therapy with methylaminolevulinate. Twenty-five patients with actinic keratoses on the head received one session of photodynamic therapy with methylaminolevulinate. Biopsies were taken at baseline and six weeks after treatment. Immuno-histochemical staining was performed for VDR, P53, Ki67 and -catenin. Basal serum 25(OH)D levels were determined. Cases with a positive histological response to treatment had significantly higher serum 25(OH)D levels (26.96 (SD 7.49) ngr/mL) than those without response (18.60 (SE 7.49) ngr/mL) ( = 0.05). Patients with a complete clinical response displayed lower basal VDR expression (35.71% (SD 19.88)) than partial responders (62.78% (SD 16.735)), ( = 0.002). Our results support a relationship between vitamin D status and the response of actinic keratoses to photodynamic therapy with methylaminolevulinate.
在鳞状细胞癌小鼠模型中，在使用氨基乙酰丙酸（ALA）进行光动力治疗之前用骨化三醇预处理可增强肿瘤细胞死亡。我们评估了维生素D状态与光化性角化病对甲基氨基乙酰丙酸光动力治疗反应之间的关联。25名头部长有光化性角化病的患者接受了一次甲基氨基乙酰丙酸光动力治疗。在基线和治疗后六周进行活检。对维生素D受体（VDR）、P53、Ki67和β-连环蛋白进行免疫组织化学染色。测定基础血清25（OH）D水平。对治疗有组织学阳性反应的病例血清25（OH）D水平（26.96（标准差7.49）ngr/mL）显著高于无反应者（18.60（标准误7.49）ngr/mL）（P = 0.05）。完全临床缓解的患者基础VDR表达（35.71%（标准差19.88））低于部分缓解者（62.78%（标准差16.735）），（P = 0.002）。我们的结果支持维生素D状态与光化性角化病对甲基氨基乙酰丙酸光动力治疗反应之间的关系。
