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BIG 基因控制拟南芥茎尖分生组织的大小。

The BIG gene controls size of shoot apical meristems in Arabidopsis thaliana.

机构信息

State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, 271018, Shandong, China.

出版信息

Plant Cell Rep. 2020 Apr;39(4):543-552. doi: 10.1007/s00299-020-02510-6. Epub 2020 Feb 6.

Abstract

BIG regulates the shoot stem cell population. The shoot apical meristem (SAM) contains a population of self-renewing cells, and provides daughter cells for initiation and development of aerial parts of plants. However, the underlying mechanisms of SAM size regulation remain largely unclear. Here, we identified a mutant that displayed a large SAM, designated big-shoot meristem (big-m), in Arabidopsis thaliana. The phenotype of big-m is caused by a new T-DNA insertion allele of BIG, causing a loss of function. The big-m mutant had more stem cells in the SAM than in the wild type. Expression of WUSCHEL (WUS) and SHOOTMERISTEMLESS (STM) was promoted in big-m compared with the wild type, showing that BIG functions upstream of WUS and STM. Therefore, BIG is an important regulator of the stem cell population in the SAM. Furthermore, genetic analysis indicated that BIG acts synergistically with PIN-FORMED1 (PIN1) in controlling SAM size. Our results suggest that BIG plays an important role in controlling Arabidopsis thaliana SAM growth via PIN1-mediated auxin homeostasis.

摘要

BIG 调控着茎干细胞群体。茎尖分生组织(SAM)包含一群自我更新的细胞,为植物地上部分的起始和发育提供子细胞。然而,SAM 大小调节的潜在机制在很大程度上仍不清楚。在这里,我们鉴定了一个拟南芥突变体,其表现出大的茎尖分生组织(big-shoot meristem,big-m),命名为 big-m。big-m 的表型是由 BIG 的一个新的 T-DNA 插入等位基因引起的,导致功能丧失。与野生型相比,big-m 突变体在 SAM 中有更多的干细胞。与野生型相比,WUSCHEL(WUS)和 SHOOTMERISTEMLESS(STM)的表达在 big-m 中被促进,表明 BIG 在上游作用于 WUS 和 STM。因此,BIG 是 SAM 中干细胞群体的重要调节因子。此外,遗传分析表明,BIG 在控制 SAM 大小方面与 PIN-FORMED1(PIN1)协同作用。我们的结果表明,BIG 通过 PIN1 介导的生长素稳态在控制拟南芥 SAM 生长中发挥重要作用。

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