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IMP synthesis using immobilized adenosine (phosphate) deaminase.

作者信息

Carvalho Júnior L B, Oliveira E A, Silva M P, Accioly L G

机构信息

Departamento de Bioquimica, Universidade Federal de Pernambuco, Cidade Universitaria, Recife, PE, Brasil.

出版信息

Appl Biochem Biotechnol. 1988 Oct;19(1):21-5. doi: 10.1007/BF02921462.

DOI:10.1007/BF02921462
PMID:3202624
Abstract

Inosinic acid (IMP) has been prepared by the deamination of adenosine monophosphate (AMP) with an immobilized adenosine (phosphate) deaminase extracted from the snail Biomphalaria glabrata. The enzyme has been immobilized in polyacrylamide beads. The preparation and characterization of this system are described.

摘要

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本文引用的文献

1
Differential spectrophotometry of purine compounds by means of specific enzymes; determination of adenine compounds.利用特定酶对嘌呤化合物进行差示分光光度法;腺嘌呤化合物的测定
J Biol Chem. 1947 Feb;167(2):445-59.
2
Adenosine- and inosine-nucleotides in the phosphorus metabolism of muscle.肌肉磷代谢中的腺苷酸和肌苷酸
Biochem J. 1942 Dec;36(10-12):729-36. doi: 10.1042/bj0360729.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.