Koshiba H, Ishii Y, Yamanaka N, Kikuchi K
J Immunol. 1977 Feb;118(2):625-9.
A new type of differentiation antigens on human T cells was demonstrated by using a heterologous anti-human T cell serum (ATS). This type of antigen, referred to as human peripheral T cell antigen (HPTA), was found on peripheral T cells and medullary thymocytes, but not on cortical thymocytes and B cells. The percentage of ATS-reactive lymphocytes in human peripheral lymphoid organs was correlated with that of cells rosetting with sheep erythrocytes, but contrasted with the number of B cells defined by the presence of a complement (C) receptor or by rabbit anti-human B cell serum (ABS). ATS also reacted with T cells purified by nylon fiber column filtration but ABS did not. Chronic lymphocytic leukemia cells rosetted with either sheep erythrocytes or erythrocyte-antibody-complement complexes were lysed by ATS and ABS, respectively. Mitogenic responses of blood lymphocytes to phytohemagglutinin (PHA) and concanavalin-A (Con A) were abrogated by treating them with ATS and C, whereas ABS suppressed only their response to Con A. Although numerous thymus cells rosetted with SRBC, only 14% were reactive with ATS. Quantitative absorption studies demonstrated that HPTA content of the thymus cells was much lower than that of lymph node cells. Anatomical localization of ATS-reactive lymphocytes in human lymphoid organs studied by immunofluorescence indicated that they were present in the thymus-dependent paracortical areas of lymph node and in the medullary region of thymus. ABS, on the other hand, did not stain thymocytes but reacted selectively with the cells located in the lymphoid follicles of lymph node. These data, together with that from cell suspension studies, confirmed that HPTA were shared between medullary thymocytes and peripheral T cells.
利用一种异种抗人T细胞血清（ATS）证实了人T细胞上一种新型分化抗原的存在。这种抗原类型被称为人外周T细胞抗原（HPTA），在外周T细胞和胸腺髓质细胞上被发现，但在胸腺皮质细胞和B细胞上未发现。人外周淋巴器官中对ATS有反应的淋巴细胞百分比与与绵羊红细胞形成花环的细胞百分比相关，但与由补体（C）受体的存在或兔抗人B细胞血清（ABS）所定义的B细胞数量形成对比。ATS也与通过尼龙纤维柱过滤纯化的T细胞发生反应，但ABS不与之反应。分别用ATS和ABS裂解与绵羊红细胞或红细胞 - 抗体 - 补体复合物形成花环的慢性淋巴细胞白血病细胞。用ATS和C处理血液淋巴细胞后，其对植物血凝素（PHA）和刀豆球蛋白A（Con A）的促有丝分裂反应被消除，而ABS仅抑制其对Con A的反应。尽管大量胸腺细胞与SRBC形成花环，但只有14%与ATS反应。定量吸收研究表明，胸腺细胞的HPTA含量远低于淋巴结细胞。通过免疫荧光研究人淋巴器官中对ATS有反应的淋巴细胞的解剖定位表明，它们存在于淋巴结的胸腺依赖副皮质区和胸腺的髓质区。另一方面，ABS不使胸腺细胞染色，但与位于淋巴结淋巴滤泡中的细胞选择性反应。这些数据，连同细胞悬液研究的数据，证实了HPTA在胸腺髓质细胞和外周T细胞之间是共享的。
