Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt.
Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt.
Biol Trace Elem Res. 2020 Nov;198(1):283-292. doi: 10.1007/s12011-020-02068-0. Epub 2020 Feb 5.
The present investigation aimed to evaluate the influence of copper nanoparticles (Cu-NPs) on the growth, immunity, and oxidation resistance of common carp (3.02 ± 0.01 g, initial mean weight ± S.E.). Five groups of fish fed diets with Cu-NPs at 0, 0.5, 1, 2, and 4 mg/kg for 8 weeks. The results suggested that Cu-NPs in diets increased the growth performance and reduced FCR with linear and quadratic model (P < 0.05). Also, common carp fed Cu-NPs showed increased carcass protein, lipid, and ash contents in a dose-dependent manner (P < 0.05). The Cu accumulation in the carcass, liver, muscle, and gills increased by Cu-NPs and showed the maximum at 4 mg Cu-NPs/kg (P < 0.05). No significant alterations were found in the blood variables due to Cu-NP supplementation except for the Hb, RBCs, total protein, albumin, and globulin levels which showed the highest level in 2 mg/kg (P < 0.05). IgM level, phagocytic, lysozyme, SOD, CAT, and GPX activities were boosted by Cu-NPs with decreased malondialdehyde (MDA) content (P < 0.05). Based on regression analysis, the requirement of dietary Cu-NPs for common carp was estimated to be 2.19 to 2.91 mg/kg diet.
本研究旨在评估铜纳米粒子（Cu-NPs）对鲤鱼（初始平均体重 3.02±0.01g，初始平均体重±S.E.）生长、免疫和抗氧化能力的影响。将五组鱼分别投喂添加 0、0.5、1、2 和 4mg/kg Cu-NPs 的饲料 8 周。结果表明，饲料中的 Cu-NPs 呈线性和二次模型增加了生长性能和降低了饲料转化率（P<0.05）。此外，鲤鱼摄食 Cu-NPs 后，胴体蛋白质、脂肪和灰分含量呈剂量依赖性增加（P<0.05）。Cu-NPs 使鲤鱼的胴体、肝脏、肌肉和鳃中的 Cu 积累增加，在 4mg/kg Cu-NPs 时达到最大值（P<0.05）。除血红蛋白（Hb）、红细胞（RBCs）、总蛋白、白蛋白和球蛋白水平在 2mg/kg 时达到最高水平（P<0.05）外，Cu-NP 补充对血液变量没有显著影响。IgM 水平、吞噬作用、溶菌酶、SOD、CAT 和 GPX 活性均因 Cu-NPs 而提高，丙二醛（MDA）含量降低（P<0.05）。基于回归分析，估计鲤鱼对饲料中 Cu-NPs 的需求量为 2.19 至 2.91mg/kg 饲料。
