Viljanen M K, Granfors K, Toivanen P
J Immunol Methods. 1977;14(2):111-22. doi: 10.1016/0022-1759(77)90002-3.
A radioimmunological method for the quantitation of class-specific antibodies has been developed. The method allows the quantitation of nanogram per ml concentrations of IgG- and IgM-anti-DNP antibodies without any physical or chemicadioimmunoassay of class-specific antibodies (RIACA) against BSA (Viljanen et al., 1975) developed recently by us. DNP was coupled covalently to a cyanogen bromide activated paper disk with the augmentation of lysine molecule. Anti-DNP antibodies were allowed to react with the coupled DNP and then quantitated by their capacity to bind 125I-labeled anti-chicken-mu or anti-chicken-gamma. The inter-assay variation coefficients ranged from 8.1 to 14.7% and the mean standard deviations of duplicate determinations were about 11%. The combination of this method with the exact immunoradiometric quantitation of the total serum IgM and IgG, and with an immunoabsorption technique, makes possible to quantitate class-specific antibodies in weight units.
已开发出一种用于定量特异性类别抗体的放射免疫方法。该方法能够定量每毫升纳克浓度的IgG和IgM抗DNP抗体，而无需对我们最近开发的针对牛血清白蛋白的特异性类别抗体进行任何物理或化学放射免疫分析（RIACA）（Viljanen等人，1975年）。通过赖氨酸分子的增加，将DNP共价偶联到溴化氰活化的纸盘上。使抗DNP抗体与偶联的DNP反应，然后通过它们结合125I标记的抗鸡μ或抗鸡γ的能力进行定量。测定间变异系数范围为8.1%至14.7%，重复测定的平均标准差约为11%。该方法与血清总IgM和IgG的精确免疫放射定量以及免疫吸收技术相结合，使得以重量单位定量特异性类别抗体成为可能。
