State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People's Republic of China.
The 908th Hospital of Chinese People's Liberation Army Joint Logistic Support Force, Nanchang, People's Republic of China.
J Appl Microbiol. 2020 Jun;128(6):1764-1775. doi: 10.1111/jam.14605. Epub 2020 Feb 20.
The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy postweaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO).
Of the 128 isolates, Lactobacillus plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. Lactobacillus plantarum BLPL03 was sensitive to β-lactams, macrolides, amphenicols and cephalosporins, whereas it displayed the steady resistance to aminoglycosides, tetracyclines, quinolones and peptide antibiotics. In vitro analysis of antibacterial activities showed that L. plantarum BLPL03 inhibited the four common food-borne pathogenic bacteria including Escherichia coli O157:H7 CMCC 44828, Salmonella Typhimurium ATCC 13311, Staphylococcus aureus CMCC 26003 and Listeria monocytogenes CMCC 54007 in synergy with nZnO. Furthermore, the quantitative polymerase chain reaction test demonstrated that the combined administration of L. plantarum BLPL03 fermentation liquor (LFL) and nZnO synergistically elevated the faecal number of Bifidobacterium by 73·19-fold, and reduced the two potential enteropathogenic bacteria Enterobacteriaceae and Clostridium perfringens in mice challenged with Salm. Typhimurium. Finally, dietary supplementation with low dose of nZnO (20 mg kg ) when combined with LFL administration enhanced final body weight, fur appearance and average daily gain, and decreased feed conversion ratio and diarrhoea incidence in weaned piglets. The faecal Bifidobacterium and Lactobacillus of piglets were dramatically enhanced by 81·96- and 3·15-fold, respectively, after administration of a mixture of nZnO and LFL. Meanwhile, combination of nZnO with LFL resulted in low levels of Bacteroides, Enterococcus, and Enterobacteriaceae.
A combination of nZnO and LFL exhibits potential health-benefit properties for the control of gut microbial composition by their synergistic antimicrobial and probiotic effects.
This study may provide a potential nutritional strategy to improve performance and gut health of animals with gut microbiota disorders caused by pathogen infections and weanling, and so on.
本研究旨在从健康断奶仔猪的胃肠道中分离出乳酸菌，并研究其与氧化锌纳米粒子（nZnO）的协同抗菌和益生菌作用。
在 128 株分离株中，根据其出色的耐酸和耐胆盐特性，选择了植物乳杆菌 BLPL03。植物乳杆菌 BLPL03 对β-内酰胺类、大环内酯类、酰胺醇类和头孢菌素类敏感，而对氨基糖苷类、四环素类、喹诺酮类和肽类抗生素表现出稳定的耐药性。体外抗菌活性分析表明，植物乳杆菌 BLPL03 与 nZnO 协同抑制了包括大肠杆菌 O157:H7 CMCC 44828、鼠伤寒沙门氏菌 ATCC 13311、金黄色葡萄球菌 CMCC 26003 和单核细胞增生李斯特菌 CMCC 54007 在内的 4 种常见食源性病原体。此外，定量聚合酶链反应试验表明，植物乳杆菌 BLPL03 发酵液（LFL）和 nZnO 的联合给药可使双歧杆菌的粪便数量协同增加 73.19 倍，并降低了沙门氏菌攻毒小鼠中的潜在肠道致病菌肠杆菌科和产气荚膜梭菌。最后，当低剂量 nZnO（20mgkg）与 LFL 联合添加到饮食中时，可提高断奶仔猪的最终体重、皮毛外观和平均日增重，并降低饲料转化率和腹泻发生率。nZnO 和 LFL 混合物给药后，仔猪粪便中的双歧杆菌和乳酸菌分别显著增加了 81.96 倍和 3.15 倍。同时，nZnO 与 LFL 联合使用可降低拟杆菌、肠球菌和肠杆菌科的水平。
nZnO 和 LFL 的组合通过其协同的抗菌和益生菌作用，显示出控制肠道微生物组成的潜在健康益处。
本研究可为控制由病原体感染和断奶等引起的肠道微生物群紊乱的动物的生产性能和肠道健康提供一种潜在的营养策略。
