Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, Algeria.
EA 7394 Institut Charles Viollette, Université Lille, 59000 Lille, France.
Benef Microbes. 2020 Mar 27;11(2):163-173. doi: 10.3920/BM2019.0116. Epub 2020 Mar 5.
This study reports the probiotic attributes of strains isolated from chicken faeces and mainly their capabilities to prevent infectious diseases and improve chicken production performance. Thus, 22 strains were isolated from 50 chickens' faeces samples and assessed for their resistance to gastric acidity (pH 0.5, 1, 1.5, 2 and 2.5), tolerance to bile salts, adherence to broiler intestinal cells and antibacterial activity. These screening analyses revealed S22 and S27 as the only strains capable to survive at pH 2.0 in MRS broth (log cfu/ml=5.02 and 8.46 log respectively), while the remaining strains were not resistant to pH≤2.0. Similarly, 21 strains were resistant to bile at 0.5% (log cfu/ml=0.09-3.32 log), but only S26, S22 and S27 were able to grow in the presence of 0.1% (w/v) bile (8.23±0.15; 8.39±0.17 and 8.57±0.07 respectively). Most of these isolates (19/22) were active against ATCC 25922, SL2016 and CIP 81-3. Lactic acid is likely the main antibacterial compound produced since the neutralised supernatant was devoid of any antibacterial activity characterisation of these 22 novel strains, based on the aforementioned criteria revealed S27 as the most suitable strain for analyses. To this end, this strain was assessed for its sensitivity to different antibiotics and adhesion to poultry intestinal cells to ascertain it probiotic attributes. The administration of S27 to the chicks at 10 cfu/ml permitted to improve the animal food intake and weight. Taken together, data from and analyses indicated that S27 might be a worthy probiotic for chickens rather than adding antibiotics to animals feeding.
本研究报告了从鸡粪便中分离出的菌株的益生菌特性，主要是它们预防传染病和提高鸡生产性能的能力。因此，从 50 只鸡的粪便样本中分离出 22 株，并评估了它们对胃酸（pH0.5、1、1.5、2 和 2.5）的抗性、耐受胆盐、对肉鸡肠道细胞的粘附性和抗菌活性。这些筛选分析表明，S22 和 S27 是唯一能够在 MRS 肉汤中在 pH2.0 下存活的菌株（log cfu/ml=5.02 和 8.46log 分别），而其余菌株对 pH≤2.0 没有抗性。同样，21 株对 0.5%（w/v）胆盐有抗性（log cfu/ml=0.09-3.32log），但只有 S26、S22 和 S27 能够在 0.1%（w/v）胆盐存在下生长（8.23±0.15；8.39±0.17 和 8.57±0.07 分别）。这些分离株中的大多数（19/22）对 ATCC25922、SL2016 和 CIP81-3 具有活性。由于中和上清液中没有任何抗菌活性，因此推测乳酸是产生的主要抗菌化合物。基于上述标准对这 22 株新分离株进行了分析，结果表明 S27 是最适合分析的菌株。为此，评估了该菌株对不同抗生素的敏感性和对禽类肠道细胞的粘附性，以确定其益生菌特性。以 10 cfu/ml 的剂量给小鸡服用 S27，可以提高动物的采食量和体重。综合来看，和分析的数据表明，S27 可能是一种有价值的鸡用益生菌，而不是在动物饲料中添加抗生素。
