Yang Qi-Chao, Li Hao, Xiao Yao, Wu Cong-Cong, Yang Shao-Chen, Sun Zhi-Jun
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine, Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
Department of Oral Maxillofacial-Head Neck Oncology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
Eur J Oral Sci. 2020 Feb;128(1):37-45. doi: 10.1111/eos.12673. Epub 2020 Feb 6.
Inositol polyphosphate 4-phosphatase type II (INPP4B) is a phosphoinositide phosphatase that plays complex roles in the pathogenesis of different tumors. We aimed to explore the expression, clinicopathological significance, and prognostic value of INPP4B in oral squamous cell carcinoma (OSCC). Tissue microarrays that included samples from 176 primary OSCCs, 42 normal mucosae, and 69 dysplastic tissues were used for immunostaining analyses of INPP4B protein. Aperio ScanScope CS scanner and aperio quantification software were used to scan the microarrays and score the staining, respectively. We also evaluated the correlation between INPP4B expression and clinical parameters, pathological grades, node-positive status, and immune-related markers. Expression of INPP4B was statistically significantly upregulated in human primary OSCC tissues compared with dysplastic and normal tissues. Additionally, we found that patients with strong expression of INPP4B had a statistically significantly poorer overall survival than patients with weak expression of INPP4B. Furthermore, our study indicated that expression of INPP4B in OSCC was positively associated with expression of p-S6 , p-CAD , and certain immune checkpoints (B7-H4, Galectin-9). Therefore, INPP4B may be an independent prognostic indicator for patients with OSCC, in which it might function as an oncoprotein.
II型肌醇多磷酸4-磷酸酶（INPP4B）是一种磷酸肌醇磷酸酶，在不同肿瘤的发病机制中发挥着复杂的作用。我们旨在探讨INPP4B在口腔鳞状细胞癌（OSCC）中的表达、临床病理意义及预后价值。使用包含176例原发性OSCC样本、42例正常黏膜样本和69例发育异常组织样本的组织微阵列进行INPP4B蛋白的免疫染色分析。分别使用Aperio ScanScope CS扫描仪和aperio定量软件扫描微阵列并对染色进行评分。我们还评估了INPP4B表达与临床参数、病理分级、淋巴结阳性状态及免疫相关标志物之间的相关性。与发育异常和正常组织相比，INPP4B在人类原发性OSCC组织中的表达在统计学上显著上调。此外，我们发现INPP4B强表达的患者总生存期在统计学上显著低于INPP4B弱表达的患者。此外，我们的研究表明，OSCC中INPP4B的表达与p-S6、p-CAD及某些免疫检查点（B7-H4、半乳糖凝集素-9）的表达呈正相关。因此，INPP4B可能是OSCC患者的一个独立预后指标，在其中它可能作为一种癌蛋白发挥作用。
