Department of Nephrology, Institute of Nephrology of Chongqing and Kidney Center of PLA, Xinqiao Hospital, Army Medical University, Chongqing, 400037, China; Department of Respiratory Medicine, Xinqiao Hospital, Army Medical University, Chongqing, 400037, PR China.
Department of Nephrology, Institute of Nephrology of Chongqing and Kidney Center of PLA, Xinqiao Hospital, Army Medical University, Chongqing, 400037, China.
Gene Expr Patterns. 2020 Jan;35:119096. doi: 10.1016/j.gep.2020.119096. Epub 2020 Feb 3.
Glomerular capillary formation is one of the fundamental mysteries in renal developmental biology. However, there are still debates on this issue, and its detailed formation process has not been clarified. To resolve this problem, we performed antibody staining with ultra-thick section on embryonic and postnatal mouse kidneys. We obtained the expression patterns of several genes that play an important role in the development of glomerular capillaries. We found that blood vessel of the fetal kidneys expanded through proliferation and sprouting. During the comma-stage and S-shaped stage, 3-4 capillaries began to bud and migrate into the glomerular cleft, forming a capillary bed in the Bowman's capsule. Then, the capillary bed expanded into mature glomerular capillary by intussusceptive angiogenesis. The afferent and efferent arterioles were formed through pruning. The distribution of VEGFA in the nephron epithelial cells but not only in podocytes, induced multiple capillaries sprouted into the glomerular cleft. And CXCR4 played an important role in the differentiation and expansion of capillary bed into glomerular capillary. Immunofluorescence performed with ultra-thick section allowed us to investigate the development of complex structure tissues systematically and comprehensively.
肾小球毛细血管的形成是肾脏发育生物学的基本奥秘之一。然而，对于这一问题仍存在争议，其详细的形成过程尚未阐明。为了解决这一问题，我们对胚胎期和新生期小鼠肾脏进行了超厚切片的抗体染色。我们获得了几个在肾小球毛细血管发育中起重要作用的基因的表达模式。我们发现胎儿肾脏的血管通过增殖和出芽而扩张。在逗号期和 S 形期，3-4 个毛细血管开始出芽并迁移到肾小球裂陷处，在鲍曼囊内形成毛细血管床。然后，毛细血管床通过内陷性血管生成扩展成成熟的肾小球毛细血管。输入和输出小动脉通过修剪形成。VEGFA 在肾单位上皮细胞中的分布而不仅仅是在足细胞中，诱导多个毛细血管出芽到肾小球裂陷处。CXCR4 在毛细血管床分化和扩展为肾小球毛细血管中起重要作用。超厚切片的免疫荧光使我们能够系统而全面地研究复杂结构组织的发育。
