Department of Zoology, Genetics and Physical Anthropology, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Meat Technology Center of Galicia, 32900 San Cibrao das Viñas, Ourense, Spain.
Food Chem. 2020 Jun 15;315:126293. doi: 10.1016/j.foodchem.2020.126293. Epub 2020 Jan 25.
Estimates of quantitative proteomic distance between populations have not been reported to date. Here, quantitative proteomic distances between three Spanish bovine breeds (Asturiana de los Valles, AV; Retinta, RE; and Rubia Gallega, RG) were estimated from two-dimensional electrophoresis profiles of meat samples of longissimus thoracis muscle at 2 h post-mortem. Statistically significant distances were detected between AV/RG and the most genetically different RE breed, using the novel QD measure of quantitative proteomic distance. In total, 18 differentially abundant myofibrillar and sarcoplasmic proteins/isoforms contributing to proteomic distances between breeds were confidently identified by tandem mass spectrometry. The fast skeletal myosin regulatory light chain 2 followed by other five interacting proteins exhibited the most pronounced relative change between breeds. In addition, most differentially represented proteins could be associated with variations in meat tenderness. Therefore, they could be candidate biomarkers for molecular breeding programs and authentication of the three Spanish beef breeds.
迄今为止，尚未有关于群体定量蛋白质组学距离的估计报道。在这里，我们根据死后 2 小时取自背最长肌的肉样的二维电泳图谱，对 3 个西班牙牛品种（阿斯图里亚牛、RE 和拉古纳牛）进行了定量蛋白质组学距离的估计。使用新的 QD 定量蛋白质组学距离度量方法，在 AV/RG 和遗传差异最大的 RE 品种之间检测到了统计学上显著的距离。通过串联质谱法，共鉴定出 18 种差异丰富的肌原纤维和肌浆蛋白/同工型，这些蛋白/同工型对品种间的蛋白质组学距离有贡献。快速骨骼肌肌球蛋白轻链 2 及其后 5 种相互作用蛋白在品种间的相对变化最为显著。此外，大多数差异表达的蛋白与肉嫩度的变化有关。因此，它们可能是分子育种计划和三种西班牙牛肉品种鉴定的候选生物标志物。
