LEAF - Linking Landscape, Environment, Agriculture and Food Research Center, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
LEAF - Linking Landscape, Environment, Agriculture and Food Research Center, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal; Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
J Proteomics. 2024 Sep 15;307:105265. doi: 10.1016/j.jprot.2024.105265. Epub 2024 Jul 29.
Arouquesa cattle breed is an autochthonous Portuguese breed produced under a traditional mountain system that need improvement without affecting beef quality. The aim of this work is to compare the proteomics profiles of the Longissimus thoracis muscle from Arouquesa animals produced under different production systems. Sixty weaners were produced under the following systems: traditional (TF) and traditional with starter feed supplementation (TF + S1) with weaning and slaughtering at 9 months, the S1 + S2 (weaning at 5 months and grower supplement until slaughter) and two rearing periods with finishing supplementation (TF + S3 and S3). Upon slaughter, samples of L. thoracis were taken and analyzed using a shotgun proteomics workflow. Several putative markers of beef quality for the Arouquesa breed were identified: VIM, FSCN1, SERPINH1, ALDH1A1, NDUFB5, ANXA1, PDK4, CEMIP2, NDUFB9, PDLIM1, OXCT1, MYH4. These proteins are involved in actin binding, skeletal muscle development and in the mitochondrial respiratory chain and they can influence mostly meat tenderness and color. We identified specific proteins for each group related to different metabolisms involved in several aspects that affect meat quality parameters. Our results demonstrate the link between production practices and putative meat characteristics, which have the potential to improve the traceability of certified products. SIGNIFICANCE: Arouquesa breed is produced in a sustainable system using natural resources and contributing to the economy of low-populated rural regions in Northern Portugal. Besides their economic relevance, producing autochthonous breeds can counter rural depopulation and maintain local heritage. Additionally, consumer awareness about product quality is increasing and PDO products contribute to satisfying this demand. However, it is necessary to increase production so that it is possible to sell these products outside the production region. To ensure robust traceability and that PDO label characteristics are maintained despite increasing production yield, product analysis is of paramount importance. For this reason, proteomic approaches can provide insight into how production changes will affect beef quality and generate putative biomarkers of certified production systems.
阿罗奎萨牛是一种葡萄牙本土品种，在传统的山地系统下生产，需要在不影响牛肉质量的情况下进行改良。本工作旨在比较不同生产系统下生产的阿罗奎萨牛背最长肌的蛋白质组学图谱。60 头断奶仔猪在以下系统中生产：传统（TF）和传统加起始饲料补充（TF+S1），断奶和屠宰在 9 个月时进行，S1+S2（5 个月断奶和育肥补充直到屠宰）和两个育肥期加育肥补充（TF+S3 和 S3）。屠宰时，取背最长肌样品，采用 shotgun 蛋白质组学工作流程进行分析。确定了阿罗奎萨牛品种的一些潜在的牛肉质量标记物：VIM、FSCN1、SERPINH1、ALDH1A1、NDUFB5、ANXA1、PDK4、CEMIP2、NDUFB9、PDLIM1、OXCT1、MYH4。这些蛋白质参与肌动蛋白结合、骨骼肌发育和线粒体呼吸链，它们主要影响肉的嫩度和颜色。我们确定了每个组特有的与不同代谢物相关的特定蛋白质，这些代谢物涉及影响肉质参数的几个方面。我们的结果表明生产实践与潜在的肉特性之间存在联系，这有可能提高认证产品的可追溯性。意义：阿罗奎萨牛在可持续系统中生产，使用自然资源，为葡萄牙北部人口稀少的农村地区的经济做出贡献。除了它们的经济意义外，生产本土品种可以对抗农村人口减少，保持当地遗产。此外，消费者对产品质量的意识正在提高，PDO 产品有助于满足这一需求。然而，需要增加产量，以便能够在生产区域之外销售这些产品。为了确保稳健的可追溯性，并保持 PDO 标签特性，尽管产量增加，产品分析至关重要。为此，蛋白质组学方法可以深入了解生产变化将如何影响牛肉质量，并产生认证生产系统的潜在生物标志物。
