人红细胞乙酰胆碱酯酶活性的温度依赖性不受膜胆固醇富集的影响。
The temperature-dependence of human erythrocyte acetylcholinesterase activity is not affected by membrane cholesterol enrichment.
作者信息
Spinedi A, Rufini S, Luly P, Farias R N
机构信息
Department of Biology, University of Rome, Tor Vergata, Italy.
出版信息
Biochem J. 1988 Oct 15;255(2):547-51.
The temperature-dependence of both the lipid order parameter (SDPH) and acetylcholinesterase (AChE) activity from native and cholesterol-enriched human erythrocyte membranes was investigated. Cholesterol enrichment abolishes an inflection observed around 30 degrees C in the temperature-dependence of native membrane lipid order parameter, whereas the Arrhenius plot of the enzymic activity is substantially unaffected. These results support the view that the breaks in the Arrhenius plot of the enzyme activity are not related to sudden changes of bulk membrane physical state, but arise from a direct effect of temperature on enzyme conformation.
研究了天然和富含胆固醇的人红细胞膜的脂质序参数（SDPH）和乙酰胆碱酯酶（AChE）活性的温度依赖性。胆固醇富集消除了天然膜脂质序参数温度依赖性中在30℃左右观察到的拐点，而酶活性的阿伦尼乌斯图基本不受影响。这些结果支持这样一种观点，即酶活性的阿伦尼乌斯图中的断点与膜整体物理状态的突然变化无关，而是由温度对酶构象的直接影响引起的。
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