来自嗜热脂肪芽孢杆菌V的一种DNA修饰甲基化酶。
A DNA-modification methylase from Bacillus stearothermophilus V.
作者信息
Barra R, Chiong M, González E, Vásquez C
机构信息
Laboratorio de Biologia Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago.
出版信息
Biochem J. 1988 Oct 15;255(2):699-703.
Abstract
A type II modification methylase (M BstVI) was partially purified from the thermophilic bacterium Bacillus stearothermophilus V. The methylase catalyses the transfer of methyl groups from S-adenosyl-L-methionine to unmodified double-stranded DNA. The product of methylation was identified by paper chromatography as N6-methyladenine. Since M BstVI protects DNA against cleavage by BstVI and XhoI restriction endonucleases, it follows that it methylates the adenine residue in the sequence 5'-C-T-C-G-A-G-3'.
摘要
从嗜热脂肪芽孢杆菌V中部分纯化出II型修饰甲基化酶（M BstVI）。该甲基化酶催化甲基从S-腺苷-L-甲硫氨酸转移至未修饰的双链DNA。通过纸层析法鉴定出甲基化产物为N6-甲基腺嘌呤。由于M BstVI可保护DNA不被BstVI和XhoI限制性核酸内切酶切割，因此可以推断它会使序列5'-C-T-C-G-A-G-3'中的腺嘌呤残基发生甲基化。
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