College of Environment, Hohai University, 1 Xikang Road, Nanjing 210098, China.
Power China Kuminng Engineering Co., Ltd., Kuminng 650051, China.
Int J Environ Res Public Health. 2020 Feb 3;17(3):941. doi: 10.3390/ijerph17030941.
Periphyton is an effective matrix for the removal of pollutants in wastewater and has been considered a promising method of bioremediation. However, it still needs to be verified whether periphyton can maintain microbial activity and pollutant removal efficiency when dealing with the influence with complex components, and the underlying mechanisms of periphyton need to be revealed further. Herein, this study investigated the microbial growth, activity and functional responses of periphyton after removal of Cu from wastewater. Results showed that the cultivated periphyton was dominated by filamentous algae, and high Cu removal efficiencies by periphyton were obtained after 108 h treatments. Although 2 mg/L Cu changed the microalgal growth (decreasing the contents of total chlorophyll-a (Chla), the carbon source utilization and microbial metabolic activity in periphyton were not significantly affected and even increased by 2 mg/L Cu. Moreover, chemical oxygen demand (COD) removal rates were sustained after 0.5 and 2 mg/L Cu treatments. Our work showed that periphyton had strong tolerance and resistance on Cu stress and is environmentally friendly in dealing with wastewater containing heavy metals, as the microbial functions in pollutant removal could be maintained.
底栖藻类是去除废水中污染物的有效基质，被认为是一种很有前途的生物修复方法。然而，底栖藻类在应对复杂成分的影响时，是否能够保持微生物活性和污染物去除效率，仍需要进一步验证，其作用机制也需要进一步揭示。本研究考察了去除废水中的铜后底栖藻类的微生物生长、活性和功能响应。结果表明，培养的底栖藻类主要为丝状藻类，经过 108 h 的处理后，底栖藻类对铜的去除效率很高。尽管 2 mg/L 的铜改变了微藻的生长（降低了总叶绿素-a（Chla）的含量），但底栖藻类中的碳源利用和微生物代谢活性并没有受到显著影响，甚至在 2 mg/L 的铜存在下还增加了。此外，在 0.5 和 2 mg/L 的铜处理后，化学需氧量（COD）的去除率保持稳定。我们的工作表明，底栖藻类对铜胁迫具有很强的耐受性和抵抗力，在处理含有重金属的废水时是环保的，因为去除污染物的微生物功能可以得到维持。
