Department of Chemistry, College of Science, Huazhong Agricultural University, Wuhan, 430070, China.
Affiliated Hospital of Huazhong Agricultural University, Wuhan, 430070, China.
Anal Chim Acta. 2020 Mar 8;1101:129-134. doi: 10.1016/j.aca.2019.12.018. Epub 2019 Dec 17.
Carbon dots (CDs) were synthesized from p-aminophenol and ethylenediamine via one-step under mild condition and used as a sensitive fluorescent nanoprobe for the activity determination of N-acetyl-β-d-glucosaminidase (NAG). In this assay, p-nitrophenol was in situ produced from p-nitrophenyl-β-D-N-acetyl-glucosaminide, which was exclusively hydrolyzed by NAG. The UV absorption peak of p-nitrophenol (maximum at 400 nm) overlapped the excitation peak of CDs with maximum wavelength at 415 nm, which caused the fluorescence decline of CDs based on inner filter effect. The activity of NAG was determined by the fluorescence changes. The assay is highly sensitive to NAG with a low detection limit of 0.75 U L (K = 3) and showed a good linear relationship in the range from 1 to 45 U L. This CDs nanoprobe was successfully applied for the determination of NAG activity in human serum and urine samples.
碳点（CDs）由对氨基酚和乙二胺通过一步温和条件下合成，并用作 N-乙酰-β-D-氨基葡萄糖苷酶（NAG）活性测定的灵敏荧光纳米探针。在该测定法中，对硝基苯酚从 N-乙酰-β-D-氨基葡萄糖苷中就地产生，该物质仅被 NAG 水解。对硝基苯酚的紫外吸收峰（最大吸收波长为 400nm）与 CDs 的最大波长在 415nm 处的激发峰重叠，这导致基于内滤效应的 CDs 的荧光下降。NAG 的活性通过荧光变化来确定。该测定法对 NAG 具有高灵敏度，检测限低至 0.75 U L（K=3），在 1 至 45 U L 范围内呈现良好的线性关系。该 CDs 纳米探针成功应用于人血清和尿液样品中 NAG 活性的测定。
