Liu Jiaxin, Ning Keke, Fu Yao, Sun Yujie, Liang Jiangong
College of Science, Huazhong Agricultural University, Wuhan 430070, China.
College of Science, Huazhong Agricultural University, Wuhan 430070, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jun 5;294:122553. doi: 10.1016/j.saa.2023.122553. Epub 2023 Mar 4.
N-acetyl-beta-D-glucosaminidase (NAG) is an important biomarker for early clinical diagnosis of renal disease, suggesting the necessity to develop a fast and sensitive method for its detection. In this paper, we developed a fluorescent sensor based on polyethylene glycol (400) (PEG-400)-modified and HO-assisted etched sulfur quantum dots (SQDs). According to the fluorescence inner filter effect (IFE), the fluorescence of SQDs can be quenched by the p-nitrophenol (PNP) generated by NAG-catalyzed hydrolysis of p-Nitrophenyl-N-acetyl-β-D-glucosaminide (PNP-NAG). We successfully used the SQDs as a nano-fluorescent probe to detect the NAG activity from 0.4 to 7.5 U·L, with a detection limit of 0.1 U·L. Furthermore, the method is highly selective and was successfully used in the detection of NAG activity in bovine serum samples, suggesting its great application prospect in clinical detection.
N-乙酰-β-D-氨基葡萄糖苷酶（NAG）是肾脏疾病早期临床诊断的重要生物标志物，这表明有必要开发一种快速灵敏的检测方法。在本文中，我们基于聚乙二醇（400）（PEG-400）修饰且由羟基辅助蚀刻的硫量子点（SQDs）开发了一种荧光传感器。根据荧光内滤效应（IFE），NAG催化对硝基苯基-N-乙酰-β-D-氨基葡萄糖苷（PNP-NAG）水解产生的对硝基苯酚（PNP）可淬灭SQDs的荧光。我们成功地将SQDs用作纳米荧光探针来检测0.4至7.5 U·L的NAG活性，检测限为0.1 U·L。此外，该方法具有高度选择性，并成功用于牛血清样品中NAG活性的检测，表明其在临床检测中具有广阔的应用前景。
