Yanagiya Masahiro, Kitano Kentaro, Yotsumoto Takuma, Takai Daiya, Asahina Hiromichi, Nagayama Kazuhiro, Nakajima Jun
Department of Thoracic Surgery, The University of Tokyo Graduate School of Medicine, Tokyo, Japan.
Department of Clinical Laboratory, The University of Tokyo, Tokyo, Japan.
J Thorac Dis. 2019 Dec;11(12):5321-5327. doi: 10.21037/jtd.2019.11.59.
The aim of this study was to evaluate effect of normal saline flush injection into bronchus on creation of decellularized lung scaffolds.
Pigs were used: 3 lung grafts for decellularization with pre-treatment of normal saline injection into a bronchus, 3 for decellularization without pre-treatment and 3 treated as normal controls. We compared the characteristics of lung scaffolds created by each method.
The pretreatment procedure significantly reduced the DNA content of lung grafts, suggesting effective removal of cellular components. However, this pretreatment also reduced the elastin contents of lung grafts.
Considering this characteristic of saline pretreatment, we must continue to look for better methods to produce ideal decellularized lung grafts.
本研究的目的是评估向支气管内注射生理盐水冲洗对制备去细胞肺支架的影响。
使用猪：3个肺移植物用于经向支气管内注射生理盐水预处理后进行去细胞处理，3个用于未经预处理的去细胞处理，3个作为正常对照。我们比较了每种方法制备的肺支架的特征。
预处理程序显著降低了肺移植物的DNA含量，表明细胞成分被有效去除。然而，这种预处理也降低了肺移植物的弹性蛋白含量。
考虑到生理盐水预处理的这一特性，我们必须继续寻找更好的方法来制备理想的去细胞肺移植物。
