通过调控环氧化物水解酶的区域选择性，从外消旋环氧化物中单酶合成（R）-1,2-二醇。
Manipulating regioselectivity of an epoxide hydrolase for single enzymatic synthesis of (R)-1,2-diols from racemic epoxides.
机构信息
Wuxi School of Medicine, Jiangnan University, Wuxi, 214122, China.
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
出版信息
Chem Commun (Camb). 2020 Mar 3;56(18):2799-2802. doi: 10.1039/d0cc00283f.
Both the activity and regioselectivity of Phaseolus vulgaris epoxide hydrolase were remarkably improved via reshaping two substrate tunnels based on rational design. The elegant one-step enantioconvergent hydrolysis of seven rac-epoxides was achieved by single mutants, allowing green and efficient access to valuable (R)-1,2 diols with high eep (90.1-98.3%) and yields.
通过基于合理设计重塑两个底物隧道，菜豆环氧水解酶的活性和区域选择性得到了显著提高。通过单个突变体实现了七种 rac-环氧化物的优雅一步对映选择性水解，为高 ee（90.1-98.3%）和产率的有价值的（R）-1,2 二醇提供了绿色高效的途径。
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