Department of Biochemistry and Molecular Biology, Egerton University, Egerton, Kenya.
International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.
J Med Primatol. 2020 Aug;49(4):165-178. doi: 10.1111/jmp.12461. Epub 2020 Feb 6.
Simian immunodeficiency virus (SIV) naturally infects African non-human primates (NHPs) and poses a threat of transmission to humans through hunting and consumption of monkeys as bushmeat. This study investigated the as of yet unknown molecular diversity of SIV in free-ranging Chlorocebus species (African green monkeys-AGMs) and Papio anubis (olive baboons) within Mombasa, Kisumu and Naivasha urban centres in Kenya.
We collected blood samples from 124 AGMs and 65 olive baboons in situ, and detected SIV by high-resolution melting analysis and sequencing of PCR products.
Simian immunodeficiency virus prevalence was 32% in AGMs and 3% in baboons. High-resolution melting (HRM) analysis demonstrated distinct melt profiles illustrating virus diversity confirmed by phylogenetic analysis.
There is persistent evolutionary diversification of SIVagm strains in its natural host, AGMs and cross-species infection to olive baboons is occurring. Further study is required to establish pathogenesis of the diverse SIVagm variants and baboon immunological responses.
猴免疫缺陷病毒（SIV）自然感染非洲非人灵长类动物（NHPs），并通过狩猎和食用猴子作为丛林肉而对人类构成传播威胁。本研究调查了肯尼亚蒙巴萨、基苏木和奈瓦沙城市中心自由放养的绿长尾猴（非洲绿猴）和狒狒（橄榄狒狒）中 SIV 的分子多样性，这些病毒至今仍不为人知。
我们从 124 只绿长尾猴和 65 只橄榄狒狒身上采集了血液样本，通过高分辨率熔解分析和 PCR 产物测序来检测 SIV。
绿长尾猴的 SIV 感染率为 32%，而狒狒的感染率为 3%。高分辨率熔解（HRM）分析显示出不同的熔解曲线，通过系统发育分析证实了病毒的多样性。
在其自然宿主绿长尾猴中，SIVagm 株持续发生进化多样化，并且正在发生跨种感染到橄榄狒狒。需要进一步研究来确定不同 SIVagm 变异体的发病机制和狒狒的免疫反应。
