Jin M J, Rogers J, Phillips-Conroy J E, Allan J S, Desrosiers R C, Shaw G M, Sharp P M, Hahn B H
Department of Medicine, University of Alabama at Birmingham 35294.
J Virol. 1994 Dec;68(12):8454-60. doi: 10.1128/JVI.68.12.8454-8460.1994.
Many African primates are known to be naturally infected with simian immunodeficiency viruses (SIVs), but only a fraction of these viruses has been molecularly characterized. One primate species for which only serological evidence of SIV infection has been reported is the yellow baboon (Papio hamadryas cynocephalus). Two wild-living baboons with strong SIVAGM seroreactivity were previously identified in a Tanzanian national park where baboons and African green monkeys shared the same habitat (T. Kodama, D. P. Silva, M. D. Daniel, J. E. Phillips-Conroy, C. J. Jolly, J. Rogers, and R. C. Desrosiers, AIDS Res. Hum. Retroviruses 5:337-343, 1989). To determine the genetic identity of the viruses infecting these animals, we used PCR to examine SIV sequences directly in uncultured leukocyte DNA. Targeting two different, nonoverlapping genomic regions, we amplified and sequenced a 673-bp gag gene fragment and a 908-bp env gene fragment from one of the two baboons. Phylo-genetic analyses revealed that this baboon was infected with an SIVAGM strain of the vervet subtype. These results provide the first direct evidence for simian-to-simian cross-species transmission of SIV in the wild.
已知许多非洲灵长类动物自然感染了猴免疫缺陷病毒（SIV），但只有一小部分此类病毒得到了分子特征描述。黄狒狒（Papio hamadryas cynocephalus）是仅报道了SIV感染血清学证据的一种灵长类动物。此前在坦桑尼亚的一个国家公园中鉴定出两只具有强烈SIVAGM血清反应性的野生狒狒，该公园中狒狒和非洲绿猴共享同一栖息地（T. 小田、D. P. 席尔瓦、M. D. 丹尼尔、J. E. 菲利普斯 - 康罗伊、C. J. 乔利、J. 罗杰斯和R. C. 德罗西耶，《艾滋病研究与人类逆转录病毒》5:337 - 343，1989年）。为了确定感染这些动物的病毒的基因身份，我们使用聚合酶链反应（PCR）直接检测未培养白细胞DNA中的SIV序列。针对两个不同的、不重叠的基因组区域，我们从两只狒狒中的一只扩增并测序了一个673碱基对的gag基因片段和一个908碱基对的env基因片段。系统发育分析表明，这只狒狒感染了黑长尾猴亚型的SIVAGM毒株。这些结果为野生环境中SIV在猴之间的跨物种传播提供了首个直接证据。
