Department of Orthopedics Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People's Republic of China.
Department of Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
Cell Transplant. 2020 Jan-Dec;29:963689720905798. doi: 10.1177/0963689720905798.
Intervertebral disc (IVD) degeneration and consequent lower back pain is a common disease. Micro fragmented adipose tissue (MFAT) is promising for a wide range of applications in regenerative medicine. In this study, MFAT was isolated by a nonenzymatic method and co-cultured with nucleus pulposus cells (NPCs) using an indirect co-culture system . A pig disc degeneration model was used to investigate the regenerative effect of MFAT on degenerated IVDs . The mRNA expression of , , and in NPCs was significantly increased, while no significant increase was observed in the mRNA expression of proinflammatory cytokine genes after the NPCs were co-cultured with MFAT. Nucleus pulposus (NP)-specific markers were increased in MFAT cells after co-culture with NPCs. After injection of MFAT, the disc height, water content, extracellular matrix, and structure of the degenerated NP were significantly improved. MFAT promoted the matrix synthesis function of NPCs, and NPCs stimulated the NP-like differentiation of MFAT cells. In addition, MFAT also partly regenerated degenerated IVDs in the pig model.
椎间盘（IVD）退变和随之而来的下腰痛是一种常见疾病。微粉碎脂肪组织（MFAT）在再生医学的广泛应用中具有广阔的前景。在这项研究中，MFAT 是通过非酶法分离的，并通过间接共培养系统与髓核细胞（NPC）共培养。猪椎间盘退变模型用于研究 MFAT 对退变的椎间盘的再生作用。NPC 与 MFAT 共培养后，NPC 中 、 、 的 mRNA 表达显著增加，而促炎细胞因子基因的 mRNA 表达未见明显增加。与 NPC 共培养后，MFAT 细胞中 NP 特异性标志物增加。注射 MFAT 后，退变 NP 的椎间盘高度、含水量、细胞外基质和结构均有显著改善。MFAT 促进了 NPC 的基质合成功能，而 NPC 刺激了 MFAT 细胞向 NP 样分化。此外，MFAT 还部分再生了猪模型中退变的 IVD。
