长链非编码 RNA 通过海绵吸附 miR-182 抑制结直肠癌细胞的进展。

Long non-coding RNA inhibits colorectal cancer progression through sponging miR-182.

机构信息

The First Affiliated Hospital, The School of Public Health, The State Key Lab of Respiratory Disease, Guangzhou Medical University, Guangzhou, P.R. China.

Department of Gastronintestinal Surgery, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, P.R. China.

出版信息

Int J Biol Markers. 2020 Mar;35(1):10-18. doi: 10.1177/1724600819897079. Epub 2020 Feb 7.

DOI:10.1177/1724600819897079

PMID:32031046

Abstract

OBJECTIVE

Abundant evidence has illustrated that long non-coding RNA (lncRNA) plays a vital role in the regulation of tumor development and progression. Ectopic expression of a novel lncRNA, termed , has been discovered in cancers, and this lncRNA was reported to exert an anti-tumor effect. However, its biological mechanism remains unelucidated in colorectal cancer.

METHODS

A total of 159 paired colorectal cancer specimens and adjacent tissues was applied to detect the expression of by the quantitative Real-time PCR (qRT-PCR), and a series of functional assays was executed to uncover the role of this lncRNA on colorectal cancer.

RESULTS

We found that the expression of in the tumor tissues was significantly down-regulated, while compared with adjacent normal tissues (0.0115 ± 0.0718 vs. 0.0347 ± 0.157; < 0.0001). Also, was observably associated with clinical T status (r = -0.184, = 0.024). Patients with advanced T status exerted a significantly lower level of than those with early T status (20.0% vs. 40.7%, = 0.021). Over-expression of inhibited cell proliferation and migration efficiency, and induced cell cycle arrest at the G0/G1 phase, and promoted cell apoptosis. Further research proved that altered the expression of its neighbor gene, , through acting as a competing endogenous RNA for miR-182 in colorectal cancer.

CONCLUSION

has a suppressive role in colorectal cancer development via modulating , which may serve as a target for colorectal cancer diagnosis and treatment.

摘要

目的

大量证据表明，长非编码 RNA（lncRNA）在肿瘤发生和发展的调控中起着至关重要的作用。一种新型 lncRNA 的异位表达，称为，已在癌症中被发现，并且据报道该 lncRNA 发挥抗肿瘤作用。然而，其在结直肠癌中的生物学机制仍不清楚。

方法

应用定量实时 PCR（qRT-PCR）检测 159 对结直肠癌标本及相邻组织中的表达，通过一系列功能实验揭示该 lncRNA 对结直肠癌的作用。

结果

我们发现，肿瘤组织中的表达明显下调，而与相邻正常组织相比（0.0115±0.0718 比 0.0347±0.157；<0.0001）。此外，与临床 T 分期显著相关（r=-0.184，=0.024）。T 分期较晚的患者的表达水平明显低于 T 分期较早的患者（20.0%比 40.7%，=0.021）。过表达抑制细胞增殖和迁移效率，并诱导细胞周期停滞在 G0/G1 期，促进细胞凋亡。进一步研究证明，通过作为结直肠癌中 miR-182 的竞争性内源 RNA 来改变其相邻基因的表达。