School of Chemistry and Life Science, Changchun University of Technology, 2055 Yanan Street, Changchun 130012, P. R. China.
School of Life Sciences, Changchun University of Science and Technology, 7186 Weixing Street, Changchun 130022, P. R. China.
Analyst. 2020 Mar 16;145(6):2357-2366. doi: 10.1039/c9an02309g.
Fe2+ is a trace metal ion required by the human body, and its abnormal metabolism can cause serious diseases. Herein, we report the development of a highly efficient "ON-OFF" fluorescent probe based on carbon dots (CDs), prepared by a simple one-step hydrothermal method. The CDs exhibited exceptional water dispersibility and stability, superior luminescence performance and low cytotoxicity. The fluorescence could be efficiently quenched by Fe2+ through an electronic transfer process. And under the optimized experimental conditions, this probe shows excellent selectivity and high sensitivity towards Fe2+ with a detection limit of 51 nmol. More interestingly, this probe could realize the visual detection of Fe2+ when Fe3+ and Cu2+ ions were efficiently shielded by tartaric acid in the presence of ascorbic acid. Furthermore, the developed fluorescent probe has been successfully applied for the detection of Fe2+ in tap water and BSA solution as well as for the biosensing of Fe2+ in living cells.
Fe2+ 是人体所需的痕量金属离子，其代谢异常可导致严重疾病。在此，我们报道了一种基于碳点（CDs）的高效“开-关”荧光探针的开发，该探针由简单的一步水热法制备而成。CDs 表现出优异的水分散性和稳定性、优越的发光性能和低细胞毒性。荧光可以通过电子转移过程被 Fe2+ 高效猝灭。并且在优化的实验条件下，该探针对 Fe2+ 具有优异的选择性和高灵敏度，检测限低至 51nmol。更有趣的是，当存在抗坏血酸时，酒石酸可以有效屏蔽 Fe3+ 和 Cu2+ 离子，该探针可以实现 Fe2+ 的可视化检测。此外，所开发的荧光探针已成功应用于自来水中和 BSA 溶液中 Fe2+ 的检测以及活细胞中 Fe2+ 的生物传感。
