School of Medical Imaging, Xuzhou Medical University, Xuzhou 221004, China; Department of Radiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221004, China.
School of Medical Imaging, Xuzhou Medical University, Xuzhou 221004, China.
Biosens Bioelectron. 2016 Sep 15;83:229-36. doi: 10.1016/j.bios.2016.04.066. Epub 2016 Apr 22.
Highly blue luminescent nitrogen-doped carbon dots (N-CDs) with a fluorescence quantum yield of 42.3% were prepared by an efficient one-step pyrolytic route from ethylenediaminetetraacetic acid and urea. The as-synthesized N-CDs were demonstrated as an effective fluorescent probe for label-free, selective and sensitive recognition of Fe(3+) with a linear range of 0.5μM to 2mM and a detection limit of 13.6nM due to Fe(3+)-quenched fluorescence (turn-off). The quenched fluorescence could be turned on after the addition of apoferritin owing to the removal of ferric species from the surface of N-CDs by apoferritin, making complex N-CDs/Fe(3+) a selective apoferritin probe with a linear range of 0.1-25μM and a detection limit as low as 2.6nM. In addition, the application of this novel N-CDs-based probe for imaging Fe(3+) ions and apoferritin in living cells suggest that this sensing system has great potential applications in biosensing, bioimaging, and many other fields.
高度蓝色发光的氮掺杂碳点（N-CDs），荧光量子产率为 42.3%，是通过乙二胺四乙酸和尿素的高效一步热解路线制备的。所合成的 N-CDs 被证明是一种有效的荧光探针，可用于无标记、选择性和灵敏地识别 Fe(3+)，线性范围为 0.5μM 至 2mM，检测限为 13.6nM，因为 Fe(3+)会猝灭荧光（关闭）。加入脱铁铁蛋白后，猝灭荧光可以打开，因为脱铁铁蛋白将铁物种从 N-CDs 的表面去除，使复合 N-CDs/Fe(3+)成为一种选择性的脱铁铁蛋白探针，线性范围为 0.1-25μM，检测限低至 2.6nM。此外，该新型基于 N-CDs 的探针在活细胞中成像 Fe(3+)离子和脱铁铁蛋白的应用表明，该传感系统在生物传感、生物成像和许多其他领域具有巨大的应用潜力。
