Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.
Oral Dis. 2020 Jul;26(5):1086-1089. doi: 10.1111/odi.13299. Epub 2020 Feb 21.
The morbidity of bisphosphonate-related osteonecrosis of the jaw (BRONJ) is also increasing with the use of bisphosphonates (BPs). Removing affected bone accurately is the most effective treatment. This study aimed to explore the feasibility of Indocyanine green (ICG)-based Near-Infrared fluorescence (NIF) imaging to remove BRONJ affected bone. Firstly, the rat model of BRONJ was constructed. And 5 mg/kg ICG were injected via tail vein, after 12 hr, the affected and healthy bone were dissected for ICG-based NIF imaging and quantification detection of fluorescence intensity. Finally, all the bone samples were sent for further pathological examination. All the affected bone tissues in rat BRONJ model were fluorescence developed with ICG. And the fluorescence developed regions were further confirmed as affected bone tissues with pathological examination. The fluorescence intensity in affected bone tissues, adjacent, and opposite bone tissues was 1.93107 ± 2.08106, 1.19106 ± 2.33105, and 1.24106 ± 1.57105, respectively (p < .05). Conclusions It was feasible that the extent of affected bone in rat model with BRONJ could be estimated intraoperative via ICG-based NIF imaging. This novel approach would become an auxiliary method in the treatment of patients with BRONJ in the future.
骨坏死相关性颌骨骨坏死（BRONJ）的发病率也随着双膦酸盐（BPs）的使用而增加。准确切除受影响的骨头是最有效的治疗方法。本研究旨在探讨基于吲哚菁绿（ICG）的近红外荧光（NIF）成像技术在去除 BRONJ 受影响的骨中的可行性。首先，构建了 BRONJ 大鼠模型。通过尾静脉注射 5mg/kg 的 ICG，12 小时后，将受影响和健康的骨头解剖出来进行 ICG 基于 NIF 的成像和荧光强度的定量检测。最后，所有的骨头样本都被送去进一步的病理检查。大鼠 BRONJ 模型中的所有受影响的骨组织都用 ICG 荧光显影。通过病理检查，荧光显影区域被进一步确认为受影响的骨组织。受影响的骨组织、相邻骨组织和对侧骨组织的荧光强度分别为 1.93107±2.08106、1.19106±2.33105 和 1.24106±1.57105（p<0.05）。结论 基于 ICG 的 NIF 成像技术可在术中估计 BRONJ 大鼠模型中受影响骨的范围。这种新方法将来可能成为治疗 BRONJ 患者的辅助方法。
