J Am Soc Mass Spectrom. 2020 Feb 5;31(2):458-462. doi: 10.1021/jasms.9b00009. Epub 2019 Dec 24.
The development of native mass spectrometry (MS) has provided structural biologists an additional tool to probe the structures of large macromolecular systems. Surface-induced dissociation (SID) is one activation method used within tandem MS experiments that has proven useful in interrogating the connectivity and topology of biologically-relevant protein complexes. We present here the use of a tilted surface and ion carpet array within a new SID device design, enabling decreased dimensions along the ion path and fewer lenses to tune. This device works well in fragmenting ions of both low (peptides) and high (protein complexes) . Results show that the ion carpet array, while enabling simplification of the back-end of the device, has deficiencies in product collection and subsequently signal at higher SID energies when fragmenting protein complexes. However, the use of the tilted surface is advantageous as an effective way to shorten the device and reduce the number of independent voltages.
本土质谱（MS）的发展为结构生物学家提供了另一种工具，用于探测大型大分子系统的结构。表面诱导解离（SID）是串联 MS 实验中使用的一种激活方法，它在研究生物相关蛋白质复合物的连接和拓扑结构方面非常有用。我们在这里介绍了在新的 SID 设备设计中使用倾斜表面和离子地毯阵列，这使得沿离子路径的尺寸减小，并且需要调整的透镜更少。该设备在碎裂低（肽）和高（蛋白质复合物）离子时都能很好地工作。结果表明，虽然离子地毯阵列能够简化设备的后端，但在碎裂蛋白质复合物时，在产物收集方面存在缺陷，进而在更高的 SID 能量下信号强度降低。然而，倾斜表面的使用是一种有效的缩短设备和减少独立电压数量的方法。
